Electronic Theses and Dissertation

Ulkus traumatik merupakan ulkus yang terjadi pada mukosa mulut akibat trauma yang ditandai dengan hilangnya epitel. proses penyembuhannya berlangsung melalui fase inflamasi, proliferasi, dan remodelling. proses ini melibatkan aktivitas fibroblas dalam proses fibroplasia, sintesis matriks ekstraseluler, dan reepitelisasi. ekstrak kulit buah delima (punica granatum l.) mengandung flavonoid, alkaloid, saponin, dan tannin yang diketahui memiliki aktivitas anti-inflamasi, antibakteri, dan antioksidan, sehingga berpotensi mempercepat penyembuhan ulkus. penelitian ini bertujuan untuk mengetahui pengaruh pemberian patch ekstrak kulit buah delima terhadap jumlah fibroblas pada penyembuhan ulkus traumatik. penelitian ini merupakan penelitian eksperimental laboratoris menggunakan 30 ekor mencit jantan (mus musculus l.) yang dibagi menjadi lima kelompok: kontrol negatif (basis patch), kontrol positif (patch triamcinolone), serta tiga kelompok perlakuan patch ekstrak kulit buah delima (5%, 7,5%, dan 10%). ekstraksi kulit buah delima dilakukan dengan metode maserasi menggunakan etanol 70% dan diformulasikan menjadi sediaan patch dengan metode casting solvent. variabel yang diamati adalah jumlah fibroblas pada hari ke-3 dan ke-7 setelah aplikasi patch. hasil analisis statistik menggunakan uji one-way anova menunjukkan adanya perbedaan bermakna pada jumlah fibroblas hari ke-3 (p=0,000) dan hari ke-7 (p=0,000). hasil uji lanjutan post hoc lsd menunjukkan bahwa antar kelompok perlakuan memiliki perbedaan jumlah fibroblas yang signifikan. disimpulkan bahwa pemberian patch ekstrak kulit buah delima (punica granatum l.) berpengaruh signifikan terhadap jumlah fibroblas pada hari ke-3 dan ke-7, sehingga berpotensi mempercepat penyembuhan ulkus traumatik. konsentrasi 10% merupakan formula paling efektif terhadap jumlah fibroblas pada penyembuhan ulkus traumatik.

Traumatic ulcers are ulcers that occur on the oral mucosa due to trauma and are characterized by the loss of epithelium. The healing process occurs through the inflammatory, proliferative, and remodelling phases. This process involving fibroblast activity in fibroplasia, extracellular matrix synthesis, and reepithelialization. Pomegranate peel extract (Punica granatum L.) contains flavonoids, alkaloids, saponins, and tannins, which are known to have anti-inflammatory, antibacterial, and antioxidant activities, thus potentially accelerating ulcer healing. This study aimed to determine the effect of pomegranate peel extract patch on the number of fibroblasts during the healing of traumatic ulcers. This laboratory experimental study used 30 male mice (Mus musculus L.), divided into five groups: negative control (patch base), positive control (triamcinolone patch), and three treatment groups receiving pomegranate peel extract patch at concentrations of 5%, 7.5%, and 10%. Pomegranate peel extraction was performed using the maceration method with 70% ethanol and formulated into patch preparations using the solvent casting method. The observed variable was the number of fibroblasts on days 3 and 7 after patch application. Statistical analysis using One-Way ANOVA showed significant differences in fibroblast counts on day 3 (p=0.000) and day 7 (p=0.000). Post Hoc LSD analysis showed significant differences in fibroblast numbers among the treatment groups. It was concluded that the administration of pomegranate peel extract patch (Punica granatum L.) had a significant effect on fibroblast counts on days 3 and 7, indicating its potential to accelerate traumatic ulcer healing. The 10% concentration was the most effective formulation for increasing fibroblast numbers during traumatic ulcer healing.