Electronic Theses and Dissertation

Osteoarthritis (oa) adalah penyakit sendi degeneratif yang ditandai dengan nyeri dan kerusakan tulang rawan. penelitian ini membandingkan model jejaring farmakologi dan pathway pi3k/akt/mtor penyakit osteoarthritis antara kurkumin dan meloxicam. protein target yang berinteraksi dengan kedua senyawa dan oa diidentifikasi menggunakan swisstargetprediction dan genecards, kemudian dianalisis melalui venny, string db, gene ontology (go), dan kyoto encyclopedia of genes and genomes (kegg). hasil venny menunjukkan bahwa kurkumin memiliki 57 protein target, sementara meloxicam memiliki 67 protein target yang terlibat dalam oa. hasil go menunjukkan bahwa kurkumin memiliki 9 mekanisme keterlibatan di dalam proses biologis, sedangkan meloxicam mempunyai 6 mekanisme keterlibatan di dalam proses biologis. hasil kegg menunjukkan interaksi antara protein-protein pada jalur pensinyalan pi3k/akt/mtor penyakit oa dan kurkumin memiliki 9 protein target sedangkan interaksi protein pada jalur yang sama dengan meloxicam berinteraksi dengan 13 protein target. protein target yang diidentifikasi mcc menunjukkan kurkumin memiliki protein target pada jalur pensinyalan pi3k/akt/mtor yaitu akt1 bcl2, gsk3b, dan braf sedangkan meloxicam memiliki protein target yaitu egfr, akt1, mtor, bcl2l1, dan gsk3b pada jalur yang sama. dapat disimpulkan, terdapat perbedaan keterlibatan protein target pada proses biologis, yaitu proses biologis yang hanya dimiliki oleh protein target yang berinteraksi dengan kurkumin, yaitu keterlibatan dalam katabolisme kolagen, respons terhadap stres oksidatif, respons terhadap inflamasi, dan respons sistem imun. sedangkan pada meloxicam terdapat proses angiogenesis yang tidak dimiliki oleh efek biologis dari protein taget yang berinteraksi dengan kurkumin. dan terdapat kesamaan protein target yaitu akt1, bcl2,dan gsk3b. serta perbedaan jumlah protein target pada setiap analisisnya. kata kunci: kurkumin, model jejaring farmakologi, osteoartritis, dan pathway pi3k/akt/mtor.

Osteoarthritis (OA) is a degenerative joint disease characterized by pain and cartilage damage. This study compared the pharmacological network model and PI3K/Akt/mTor pathway of osteoarthritis disease between curcumin and meloxicam. Target proteins that interact with both compounds and OA were identified using SwissTargetPrediction and GeneCards, then analyzed through Venny, String db, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG). Venny results showed that curcumin had 57 target proteins, while meloxicam had 67 target proteins involved in OA. GO results showed that curcumin has 9 mechanisms of involvement in biological processes, while meloxicam has 6 mechanisms of involvement in biological processes. KEGG results showed the interaction between proteins in the PI3K/Akt/mTor signaling pathway of OA disease and curcumin had 9 target proteins while protein interactions in the same pathway with meloxicam interacted with 13 target proteins. The target proteins identified by MCC showed that curcumin has target proteins in the PI3K/Akt/mTor signaling pathway, namely AKT1 BCL2, GSK3B, and BRAF, while meloxicam has target proteins, namely AKT1, Mtor, BCL2L1, and GSK3B in the same pathway. It can be concluded that there are differences in the involvement of target proteins in biological processes, namely biological effects that are only owned by target proteins that interact with curcumin such as involvement in collagen catabolism, response to oxidative stress, response to inflammation, and immune system response. Whereas in meloxicam there is an angiogenesis process that is not possessed by the biological effects of taget proteins that interact with curcumin. And there are similarities in target proteins, namely AKT1, BCL2, and GSK3B. As well as differences in the number of target proteins in each analysis. Keywords: Curcumin, models network pharmacology, osteoarthritis, and pathway PI3K/Akt/mTor.