Electronic Theses and Dissertation

Sp. beds3 terhadap sel kanker payudara t47d serta mengidentifikasi kandungan metabolit sekundernya menggunakan metode gc-ms. uji sitotoksisitas dilakukan dengan metode mtt assay, dan hasilnya menunjukkan bahwa ekstrak metanol bacillus sp. beds3 memiliki efek sitotoksik dengan nilai ic50 sebesar 217,2 µg/ml, yang dikategorikan sebagai aktivitas sitotoksik sedang atau moderat. analisis gc-ms mengidentifikasi 35 senyawa dalam ekstrak, dengan kelompok senyawa dominan berupa alkaloid (49,52%), diikuti oleh asam lemak, terpenoid, fenol, alkohol, dan asam amino. senyawa dengan konsentrasi tertinggi adalah d-leucine (23,93%), diikuti oleh 1,4-diaza-2,5-dioxo-3-isobutyl bicyclo [4.3.0] nonane (14,40%) dan benzeneethanamine (8,41%), yang diketahui memiliki potensi antikanker. hasil penelitian ini menunjukkan bahwa bacillus sp. beds3 berpotensi sebagai sumber senyawa bioaktif untuk pengembangan agen antikanker alternatif. kata kunci: sitotoksisitas, t47d, mtt assay, gcms, metabolit sekunder.

This study aims to evaluate the cytotoxic effects of the methanol extract of Bacillus sp. BEDS3 on T47D breast cancer cells and to identify its secondary metabolite content using the GC-MS method. Cytotoxicity testing was conducted using the MTT assay, revealing an IC50 value of 217,2 µg/mL, indicating moderate cytotoxic activity. GC-MS analysis identified 35 compounds in the extract, with alkaloids (49.52%) as the dominant group, followed by fatty acids, terpenoids, phenols, alcohols, and amino acids. The most abundant compounds were D-Leucine (23.93%), 1,4-diaza-2,5-dioxo-3-isobutyl bicyclo [4.3.0] nonane (14.40%), and Benzeneethanamine (8.41%), which are known for their potential anticancer properties. These findings suggest that Bacillus sp. BEDS3 is a promising source of bioactive compounds for developing alternative anticancer agents. However, further research is needed to assess its effects on normal cells and to elucidate its mechanism of action in greater detail. Keywords: Cytotoxicy, T47D, MTT assay, GCMS, Secondary metabolites.