Electronic Theses and Dissertation

Siput air tawar (faunus ater) hidup di perairan dangkal berlumpur dan berpasir serta memperoleh makanan melalui mekanisme filter feeder. kondisi tersebut memungkinkan tubuh siput mengandung berbagai jenis bakteri yang dapat menyebabkan mencemari organisme dan lingkungan. penelitian ini bertujuan untuk mengidentifikasi keberadaan shigella sp. pada faunus ater menggunakan analisis gen 16s rrna. metode penelitian dilakukan secara observasi dan eksplorasi laboratorium menggunakan metode carter dimodifikasi dan analisis gen 16s rrna. hasil penelitian menunjukkan secara metode carter adanya pertumbuhan bakteri berupa kekeruhan pada media bhib, terdapat koloni berwarna bening dan berbentuk bulat pada media ssa, secara pewarnaan gram menunjukkan bakteri gram negatif berbentuk batang pendek. hasil pengamatan secara analisis gen 16s rrna menunjukkan hasil isolat memiliki kemiripan sebesar 98,35% dengan bakteri shigella sp. dapat disimpulkan bahwa hasil isolat bakteri dari siput air tawar merupakan bakteri gram negatif yang secara mikrobiologi sederhana, dan termasuk ke dalam genus bakteri shigella berdasarkan analisis gen 16s rrna.

The fresh water snail Faunus ater inhabits shallow muddy and sandy waters, obtaining food through a filter-feeding mechanism. These conditions allow the snail's body to harbor various types of bacteria that may contaminate organisms and the environment. This study aimed to identify the presence of Shigella sp. in Faunus ater using 16S rRNA gene analysis. The research methods involved observation and laboratory exploration using a modified Carter method and 16S rRNA gene analysis. The study results indicated bacterial growth through the Carter method, evidenced by turbidity in BHIB media, clear, round colonies on SSA media, and Gram staining that showed Gram negative, short rod-shaped bacteria. The 16S rRNA gene analysis results revealed that the isolate had a similarity of 98,35% to Shigella sp. It can be concluded that the bacterial isolate from the freshwater snail is Gram-negative and, based on simple microbiological characterization and and belongs to the genus Shigella based on 16S rRNA gene analysis.