Electronic Theses and Dissertation

Kitosan merupakan polimer alami yang dapat ditemukan pada kelompok hewan sefalopoda, seperti tulang sotong (sepia officinalis). kitosan bersifat biokompatibel, bioadhesi, biodegradasi, tidak toksik serta memiliki aktivitas antibakteri dan antioksidan yang banyak diaplikasikan di bidang kedokteran gigi. enterococcus faecalis merupakan patogen resisten di rongga mulut yang sering ditemukan pada infeksi sekunder perawatan saluran akar. kemampuan e. faecalis dalam melawan stres oksidatif dapat mengganggu keberhasilan terapi endodontik sehingga eliminasi bakteri ini sangat diperlukan. tujuan penelitian ini adalah untuk mengetahui efek larutan kitosan dari tulang sotong (s. officinalis) terhadap kerusakan oksidatif sel e. faecalis. penelitian ini merupakan penelitian eksperimental laboratoris dengan rancangan penelitian post-test only control group design menggunakan 6 kelompok perlakuan yaitu larutan kitosan 0,5%, 1%, 2%, dan 3%, chlorhexidine sebagai kontrol positif, dan aquades sebagai kontrol negatif. parameter yang diamati meliputi kadar superoxide dismutase (sod) dan kerusakan oksidatif sel bakteri setelah 24, 48 dan 72 jam inkubasi. sebelum dilakukan uji kerusakan oksidatif dan uji kadar sod, terlebih dahulu dilakukan uji karakterisasi dari kitosan tulang sotong (s. officinalis). hasil penelitian diperoleh karakteristik kitosan sebagai berikut: rendemen transformasi kitin menjadi kitosan sebesar 10%, kadar air 3,262%, larut dalam asam asetat 1%, dan memiliki derajat deasetilasi sebesar 57%. pada hasil uji kadar sod dan kerusakan oksidatif menunjukkan bahwa larutan kitosan konsentrasi 1% secara signifikan menurunkan kadar sod dan menyebabkan kerusakan oksidatif pada aktivitas protein sel e. faecalis pada waktu inkubasi 24 jam. kesimpulan, larutan kitosan tulang sotong (sepia officinalis) memiliki efek dalam menghambat pertumbuhan bakteri enterococcus faecalis namun tidak menyebabkan kematian sel. kata kunci: kitosan, tulang sotong, enterococcus faecalis, kerusakan oksidatif, superoxide dismutase (sod)

Chitosan is a natural polymer that can be found in groups of cephalopod animals, such as cuttlefish bones (Sepia officinalis). Chitosan is biocompatible, bioadhesion, biodegradable, non-toxic and has antibacterial and antioxidant activities that are widely applied in the field of dentistry. Enterococcus faecalis is a resistant pathogen in the oral cavity that is often found in secondary infections of root canal treatment. The ability of E. faecalis to resist oxidative stress can interfere with the success of endodontic therapy so that the elimination of these bacteria is very necessary. The purpose of this study was to determine the effect of chitosan solution from cuttlefish bone (S. officinalis) on oxidative damage to E. faecalis cells. This study is a laboratory experimental study with a post-test only control group design using 6 treatment groups, namely 0.5%, 1%, 2%, and 3% chitosan solution, chlorhexidine as a positive control, and distilled water as a negative control. Parameters observed included Superoxide Dismutase (SOD) levels and oxidative damage to bacterial cells after 24, 48 and 72 hours of incubation. Prior to the oxidative damage test and SOD level test, characterization test of cuttlefish bone (S. officinalis) chitosan was conducted. The results of the study obtained the following chitosan characteristics: The yield of chitin transformation into chitosan is 10%, the water content is 3.262%, soluble in 1% acetic acid, and has a deacetylation degree of 57%. In the test results of SOD levels and oxidative damage showed that 1% concentration chitosan solution significantly reduced SOD levels and caused oxidative damage to the protein activity of E. faecalis cells at 24 hours incubation time. In conclusion, cuttlefish bone (Sepia officinalis) chitosan solution has an effect in inhibiting the growth of Enterococcus faecalis bacteria but does not cause cell death. Keywords: Chitosan, Cuttlefish Bone, Enterococcus faecalis, Oxidative Damage, Superoxide Dismutase (SOD)