Electronic Theses and Dissertation

Ikan seurukan (osteochilus jeruk) merupakan salah satu jenis ikan air tawar natif indonesia, pelestarian dan peningkatan produksi ikan seurukan dari kegiatan budidayaperlu dilakukan mengingat jumlahnya yang semakin menurun di alam, sehingga teknologi pembenihan ikan seurukan perlu dikembangkan untuk menyediakan bibit berkualitas dalam jumlah yang cukup sepanjang tahun. penelitian ini bertujuan untuk mengetahui pengaruh perbedaan jenis pengencer (ekstender) terhadap kualitas sperma ikan seurukan. ikan uji yang digunakan berjumlah 30 ekor. rancangan yang digunakan pada penelitian ini yaitu rancangan acak lengkap (ral) dengan 4 perlakuan 4 kali ulangan. faktor yang diuji adalah perbedaan jenis ekstender, yaitu larutan ringer,nacl fisiologis, glucose base dan larutan urea. hasil penelitian menunjukkan bahwa media glucose base mampu mempertahankan persentase motilitas (67,25 ± 5,31b), viabilitas (55,50 ± 7,04c), fertilisasi (89,± 6,65b), derajat penetasan (77,00 ±7,52c) dan kelangsungan hidup larva (77,00 ± 7,52c) selama penyimpanan 0, 6 dan 12jam. bedasarkan penelitian dapat disimpulkan bahwa media pengencer glucose basemerupakan media pengencer terbaik dalam mempertahankan kualitas sperma ikan seurukan selama penyimpanan. kata kunci: ikan seurukan, media pengencer, motilitas sperma, viabilitas sperma

Seurukan fish (Osteochilus jeruk) is a type of freshwater fish native to Indonesia,preserving and increasing the production of Seurukan fish from cultivation activities needs to be carried out considering that their numbers are decreasing in nature, so seeding technology for Seurukan fish needs to be developed to provide quality seeds in sufficient quantities. throughout the year. This research aims to determine the effect of different types of diluents (extenders) on the quality of Seurukan fish sperm. There were 30 test fish used. The design used in this research was a Completely Randomized Design (CRD) with 4 treatments and 4 replications. The factors tested were different types of extenders, namely Ringer's Solution, Physiological NaCL, Glucose base and Urea Solution. The results showed that the Glucose Base media was able to maintain the percentage of motility (67.25 ± 5.31b), viability (55.50 ± 7.04c), fertilization (89.25 ±6.65b), hatching degree (77.00 ± 7.52c) and larval survival (77.00 ± 7.52c) during 0, 6 and 12 hours of storage. Based on research, it can be concluded that Glucose Basediluent media is the best diluent media in maintaining the quality of Seurukan fish sperm during storage. Keywords: Seurukan fish, diluent media, sperm motility, sperm viability