Electronic Theses and Dissertation

Telah dilakukan penelitian tentang uji aktivitas anti uv ekstrak metanol teripang hitam (holothuria atra). tujuan penelitian ini adalah untuk menentukan adanya aktivitas anti uv ekstrak metanol teripang hitam (holothuria atra). guna untuk memberikan informasi ilmiah terkait potensi ekstrak teripang hitam (holothuria atra) sebagai anti uv. pengambilan sampel teripang hitam (holothuria atra) diambil di pantai benteng inong balee, desa lamreh, kecamatan masjid raya, kabupaten aceh besar. ekstraksi teripang hitam (holothuria atra) diekstrak dengan pelarut metanol menggunakan metode maserasi selama 3x24 jam. metabolit sekunder yang dikandung teripang hitam (holothuria atra) dapat menentukan adanya kandungan total fenolik, penentuan total senyawa fenolik menggunakan metode folin-ciocalteu dengan kurva asam galat. analisis gc-ms yaitu mengidentifikasi jenis dan jumlah senyawa zat aktif anti uv pada sampel dan menentukan nilai spf menggunakan metode spektrofotometri uv-vis. hasil penelitian yang didapatkan menunjukkan rendemen ekstrak dari proses ekstraksi maserasi sebesar 2,125 %. pengujian kadar total fenolik telah menunjukkan adanya senyawa total fenolik pada sampel sebesar 752 µggae/g ekstrak. analisis gc-ms telah menunjukkan adanya 34 senyawa yang teridentifikasi dan terdapat puncak tertinggi yang dominan terhadap anti uv yaitu senyawa aktif asam 9-oktadecenoat (z)- (12,95 %), asam 9,12-oktadekadienoat (z,z)- (11,25 %) dan asam heksadekanoat (9,47 %). penentuan nilai spf kategori proteksi tertinggi diperoleh pada konsentrasi 4000 ppm sebesar 16,08755 (proteksi ultra), 1000 ppm sebesar 11,62289 ( proteksi maksimal) dan 2000 ppm sebesar 7,890219 (proteksi ekstra). kata kunci: teripang hitam, holothuria atra, metanol, spektrofotometri-uv-vis, total fenolik, gcms.

Research has been conducted on Anti-UV Activity Test of Methanol Extract of Black Sea Cucumber (Holothuria atra). The purpose of this study was to determine the anti-UV activity of methanol extract of black sea cucumber (Holothuria atra). In order to provide scientific information related to the potential of black sea cucumber extract (Holothuria atra) as anti-UV. Sampling of black sea cucumber (Holothuria atra) was taken at Benteng Inong Balee Beach, Lamreh Village, Masjid Raya District, Aceh Besar Regency. Extraction of black sea cucumber (Holothuria atra) was extracted with methanol solvent using maceration method for 3x24 hours. Secondary metabolites contained in black sea cucumber (Holothuria atra) can determine the presence of total phenolic content, Determination of total phenolic compounds using the folin-ciocalteu method with gallic acid curve. GC-MS analysis is to identify the type and amount of anti-UV active substance compounds in the sample and determine the SPF value using the UV-Vis Spectrophotometry method. The results obtained showed the extract yield from the maceration extraction process was 2,125%. The total phenolic content test showed the presence of total phenolic compounds in the sample of 752 µgGAE/g. GC-MS analysis has shown the presence of 34 identified compounds and there are the highest peaks that are dominant to anti-UV, namely active compounds 9-Octadecenoic acid (Z) (12,95%), 9,12-octadecadienoic acid (Z,Z) (11,25%) and hexadecanoic acid (9,47 %). Determination of the SPF value of the highest protection category was obtained at a concentration of 4000 ppm at 16,08755 (Ultra Protection), 1000 ppm at 11,62289 (Maximum Protection) and 2000 ppm at 7,890219 (Extra Protection). Keywords: Black sea cucumber, Holothuria atra, Methanol, UV-Vis spectrophotometry, Total phenolic, GCMS.