Electronic Theses and Dissertation

Spodoptera frugiperda (lepidoptera;noctuidae) merupakan hama invasif yang berasal dari benua amerika dan sudah menyebar di berbagai negara. hama ini bersifat polifag karena dapat menyerang berbagai jenis tanaman seperti famili tanaman. namun, serangga ini menjadi hama utama pada tanaman jagung famili poaceae. larva s. frugiperda merusak tanaman jagung mulai dari fase vegetatif hingga fase generatif. bintaro (cerbera odollam) merupakan tanaman dari famili apocynaceae dan daun sirih hutan (piper aduncum) merupakan tumbuhan dari famili piperaceae. kedua tanaman ini dapat dimanfaatkan sebagai pestisida nabati karena mengandung senyawa metabolit sekuder yang berpotensi sebagai insektisida, fungisida, bakterisida, repellent, antifeedant, oviposisi dan dapat menghambat perkembangan hidup serangga. tujuan penelitian untuk mengetahui potensi campuran ekstrak daun bintaro dan daun sirih hutan sebagai insektisida nabati terhadap s. frugiperda dilaboratorium. penelitian ini dilaksanakan di laboratorium ilmu hama tumbuhan dan laboratorium analisis pangan dan hasil pertanian fakultas pertanian universitas syiah kuala, sejak bulan november 2023 sampai juni 2024. penelitian ini menggunakan rancangan acak lengkap (ral) non faktorial yang terdiri dari 6 taraf perlakuan dengan konsentrasi 0, 10g ekstrak daun bintaro (b), 10g ekstrak daun sirih hutan (sh), 5g b + 5g sh, 7,5g b + 2,5g sh, dan 2,5g b,+ 7,5g sh dengan masing-masing 4 ulangan, sehingga terdapat 24 percobaan. peubah yang diamati meliputi daya repellent, mortalitas, antifeedant, persentase pupa terbentuk dan persentase imago yang muncul. hasil penelitian menunjukkan bahwa campuran ekstrak daun bintaro dan daun sirih hutan berpengaruh nyata terhadap daya repellent larva s. frugiperda. rata-rata daya repellent larva s. frugiperda berbeda tidak nyata antara perlakuan 10g bintaro (p1) dengan 7,5g b + 2,5g sh (p4), namun berbeda nyata dengan 2,5g b + 7,5g sh (p5), 10g sh (p2), dan 5g b + 5g sh (p3). selanjutnya campuran ekstrak daun bintaro dan daun sirih hutan berpengaruh sangat nyata terhadap mortalitas s. frugiperda. rata-rata mortalitas s. frugiperda terdapat perlakuan yang berbeda nyata baik pada pengamatan 3,4,5,6 dan 7 hsa. persentase antifeedant berpengaruh nyata terhadap s. frugiperda, aplikasi campuran ekstrak daun bintaro dan daun sirih hutan tidak berbeda nyata antara 10g b (p1) dengan 7,5g sh (p4), namun berbeda nyata dengan 10g sh (p2) dan 2,5g b + 7,5g sh (p5). perlakuan 5g b + 5g sh (p3) berbeda nyata dengan perlakuan lainnya. rata-rata persentase pupa terbentuk pada perlakuan 5g b + 5g sh(p3) tidak berbeda nyata dengan 10g sh(p2), namun berbeda nyata dengan perlakuan lainnya. kemudian rata-rata persentase imago yang muncul pada perlakuan 10g sh(p2) tidak berbeda nyata dengan perlakuan 2,5g b + 7,5g sh (p5), namun berbeda nyata dengan perlakuan 10g b (p1), 5g b + 5g sh (p3), dan 7,5g b + 2,5g sh (p4). campuran ekstrak daun bintaro dan daun sirih hutan dengan konsentrasi 5g bintaro + 5g sirih hutan, dan perlakuan tunggal dengan konsentrasi 10g sirih hutan berpengaruh terhadap daya repellent terhadap larva spodoptera frugiperda. campuran ekstrak nabati dengan konsentrasi 5g bintaro + 5g sirih hutan berpotensi terhadap mortalitas larva s.podoptera frugiperda karena mampu mematikan larva mencapai 80% pada 7 hari setelah aplikasi (hsa). campuran ekstrak daun bintaro dan daun sirih hutan dengan konsentrasi 2,5g bintaro + 7,5g sirih hutan dapat menghambat kemunculan imago s. frugiperda.

**Translation to English:** *Spodoptera frugiperda* (Lepidoptera; Noctuidae) is an invasive pest originating from the American continent and has spread to various countries. This pest is polyphagous, meaning it can attack a wide range of plant species, including various plant families. However, this insect is a major pest for corn plants in the family Poaceae. The larvae of *S. frugiperda* damage corn plants from the vegetative to the generative phase. Bintaro (*Cerbera odollam*) is a plant from the family Apocynaceae, and wild betel leaf (*Piper aduncum*) is a plant from the family Piperaceae. Both plants can be used as botanical pesticides because they contain secondary metabolite compounds with potential as insecticides, fungicides, bactericides, repellents, antifeedants, ovipositional inhibitors, and can disrupt insect development. The objective of this study is to assess the potential of a mixture of bintaro leaf extract and wild betel leaf extract as a botanical insecticide against *S. frugiperda* in the laboratory. This research was conducted in the Plant Pest Science Laboratory and the Food and Agricultural Product Analysis Laboratory, Faculty of Agriculture, Syiah Kuala University, from November 2023 to June 2024. The study used a Completely Randomized Design (CRD) with a non-factorial approach, consisting of six treatment levels with concentrations of 0, 10g bintaro leaf extract (B), 10g wild betel leaf extract (SH), 5g B + 5g SH, 7.5g B + 2.5g SH, and 2.5g B + 7.5g SH, each with four replications, resulting in 24 experiments. The observed variables included repellency, mortality, antifeedant activity, percentage of pupae formation, and percentage of emerging adults. The results showed that the mixture of bintaro leaf extract and wild betel leaf extract significantly affected the repellency of *S. frugiperda* larvae. The average repellency of *S. frugiperda* larvae was not significantly different between the 10g bintaro (P1) treatment and the 7.5g B + 2.5g SH (P4) treatment but was significantly different from the 2.5g B + 7.5g SH (P5), 10g SH (P2), and 5g B + 5g SH (P3) treatments. Furthermore, the mixture of bintaro leaf extract and wild betel leaf extract had a highly significant effect on the mortality of *S. frugiperda*. The average mortality of *S. frugiperda* showed significant differences across the 3rd, 4th, 5th, 6th, and 7th days after application (DAA). The antifeedant percentage significantly affected *S. frugiperda*, with the application of the bintaro and wild betel leaf extract mixture showing no significant difference between the 10g B (P1) treatment and the 7.5g SH (P4) treatment but significantly different from the 10g SH (P2) and 2.5g B + 7.5g SH (P5) treatments. The 5g B + 5g SH (P3) treatment was significantly different from the other treatments. The average percentage of pupae formation in the 5g B + 5g SH (P3) treatment was not significantly different from the 10g SH (P2) treatment but was significantly different from the other treatments. The average percentage of emerging adults in the 10g SH (P2) treatment was not significantly different from the 2.5g B + 7.5g SH (P5) treatment but was significantly different from the 10g B (P1), 5g B + 5g SH (P3), and 7.5g B + 2.5g SH (P4) treatments. The mixture of bintaro and wild betel leaf extracts at a concentration of 5g bintaro + 5g wild betel, and the single treatment with 10g wild betel, had an effect on repellency against *S. frugiperda* larvae. The botanical extract mixture with a concentration of 5g bintaro + 5g wild betel has potential for *S. frugiperda* larval mortality, as it was able to kill up to 80% of larvae at 7 days after application (DAA). The mixture of bintaro and wild betel leaf extracts with a concentration of 2.5g bintaro + 7.5g wild betel can inhibit the emergence of *S. frugiperda* adults.