Electronic Theses and Dissertation

Telah dilakukan sintesis nanopartikel tembaga dengan metode green synthesis menggunakan ekstrak air daun srikaya (annona squamosa l.) sebagai agen pereduksi dan stabilisator. hasil penentuan total phenolic content (tpc) pada ekstrak air daun srikaya menggunakan metode folin-ciocalteu adalah sebesar 560 gae/g dan hasil penentuan total flavonoid content (tfc) pada ekstrak air daun srikaya menggunakan metode kolorimetri alcl3 adalah sebesar 7,0 qe/g. kandungan fenolik dan falvonoid terdapat pada ekstrak air daun srikaya yang bertanggung jawab untuk pembentukan nanopartikel tembaga. hasil reaksi reduksi ekstrak air daun srikaya terhadap ion cu2+ dikonfirmasi dengan menggunakan spektrofometer uv-vis, fourier transform infrared (ft-ir), x-ray diffraction (xrd), scanning electron microscopy (sem) dan transmission electron microscopy (tem). hasil karakterisasi menunjukkan bahwa nanopartikel yang terbentuk adalah cu, cuo, cu2o dan cuo2. analisis ft-ir memperlihatkan gugus oh berperan dalam proses reduksi logam. hasil sintesis nanopartikel tembaga memiliki aktivitas antimikroba terhadap bakteri staphylococcus aureus, bakteri escherichia coli dan jamur candida albicans dengan daya hambat lemah, sementara ekstrak murni tidak menunjukkan aktivitas sama sekali. kata kunci: green synthesis, annona squamosa l., nanopartikel tembaga, aktivitas antimikroba

Synthesis of copper nanoparticles has been carried out using the green synthesis method employing water extract of sugar apple (Annona squamosa L.) leaves as a reducing agent and stabilizer. The total phenolic content (TPC) in the water extract of srikaya leaves that was determined using the Folin-Ciocalteu method was 560 GAE/g and the total flavonoid content (TFC) in the water extract of srikaya leaves that was determined using the AlCl3 colorimetric method was 7.0 QE/g . The phenolic and flavonoid content found in the water extract of srikaya leaves are responsible for the formation of copper nanoparticles. The results of the reduction reaction of srikaya leaf water extract on Cu2+ ions were confirmed using a UV-VIS spectrophotometer, Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). The characterization results show that the nanoparticles formed are Cu, CuO, Cu2O and CuO2. FT-IR analysis shows that the OH group plays a role in the metal reduction process. The obtained copper nanoparticles have antimicrobial activity against Staphylococcus aureus bacteria, Escherichia coli bacteria and Candida albicans fungi with weak inhibitory power, while the pure extract shows no activity at all. Keywords: Green synthesis, Annona squamosa L., copper nanoparticles, antimicrobial activity