Electronic Theses and Dissertation

Keberhasilan perbanyakan tanaman nilam secara in vitro sangat dipengaruhi oleh keberhasilan tahap perakaran. penelitian ini menggunakan kombinasi zat pengatur tumbuh (zpt) auksin berupa naa (naphtalene acetic acid) dan iba (indole butyric acid) serta arang aktif pada media kultur in vitro dengan tujuan untuk mengetahui kombinasi konsentrasi yang optimal dalam menginduksi pertumbuhan akar pada eksplan daun nilam aceh (pogostemon cablin benth.) secara in vitro. penelitian dilaksanakan di laboratorium fisiologi (bagian tumbuhan) fakultas matematika dan ilmu pengetahuan alam, universitas syiah kuala mulai agustus 2023 hingga maret 2024. penelitian ini menggunakan rancangan acak lengkap (ral) faktorial dengan dua faktor yaitu penambahan zpt naa 0 mg/l (kontrol), 0,01 mg/l dan 0,02 mg/l dan penambahan zpt iba 0 mg/l (kontrol), 1 mg/l, 2 mg/l, dan 3 mg/l. masing-masing perlakuan diberi penambahan arang aktif sebanyak 2 gr/l. pengamatan dilakukan selama 34 hst dengan empat parameter yaitu persentase eksplan yang hidup, persentase eksplan yang berakar, jumlah akar dan panjang akar. hasil penelitian menunjukkan bahwa kombinasi zpt naa dan iba menghasilkan 100% eksplan hidup serta semua eksplan memiliki akar pada semua perlakuan. perlakuan kombinasi konsentrasi naa dan iba berpengaruh nyata terhadap jumlah dan panjang akar. kata kunci: nilam aceh (pogostemon cablin benth.), naa, iba, arang aktif, akar.

The success of patchouli plant propagation in vitro is strongly influenced by the success of the rooting stage. This study uses a combination of auxin growth regulators (ZPT) in the form of NAA (Naphtalene Acetic Acid) and IBA (Indole Butyric Acid) and activated charcoal in in vitro culture media with the aim of knowing the optimal concentration combination for inducing root growth in aceh patchouli (Pogostemon cablin Benth.) leaf explants in vitro. The research was conducted at the Physiology Laboratory (Plant Section), Faculty of Mathematics and Natural Sciences, Syiah Kuala University, from August 2023 to March 2024. This study used a factorial completely randomized design (CRD) with two factors, namely the addition of ZPT NAA 0 mg/L (control), 0.01 mg/L, and 0.02 mg/L, and the addition of ZPT IBA 0 mg/L (control), 1 mg/L, 2 mg/L, and 3 mg/L. Each treatment was given the addition of activated charcoal, as much as 2 g/L. Observations were made during 34 HST with four parameters, namely the percentage of living explants, the percentage of rooted explants, the number of roots, and root length. The results showed that the combination of NAA and IBA ZPT produced 100% living explants, and all explants had roots in all treatments. The treatment of the combination of NAA and IBA concentrations had a significant effect on the number and length of roots. Keywords: Patchouli (Pogostemon cablin Benth.), NAA, IBA, Activated charcoal and root