Electronic Theses and Dissertation

Aktivitas antioksidan dan toksisitas ekstrak rumput laut gracilaria verrucosa telah dilakukan. penelitian ini bertujuan untuk mengetahui mengetahui bagaimana aktivitas antioksidan dan toksisitas serta komponen kimia yang terpadat dari ekstrak metanol, etil asetat dan n-heksana rumput laut gracilaria verrucosa juga untuk mengetahui besar energi gibss (∆g) dari senyawa utama masing-masing ekstrak melalui studi in silico molecular docking. rumput laut gracilaria verrucosa di maserasi menggunakan pelarut metanol. ekstrak metanol dipartisi dengan berturut-turut n-heksana dan etil asetat, sehingga diperoleh ekstrak n-heksana dan etil asetat. masing-masing ekstrak diuji aktivitas antioksidan dan toksisitasnya dengan metode 1- diphenyl-2-pycrilhidrazil (dpph) dan brine shrimp lethality test (bslt). hasil uji aktivitas antioksidan ekstrak metanol, etil asetat dan n-heksana hasil partisi menghasilkan ic50 secara berturut-turut yaitu 280,82 ppm; 137,90 ppm dan 314,51 ppm. hasil uji aktivitas toksisitas ekstrak metanol, etil asetat dan n-heksana hasil partisi menghasilkan lc50 secara berturut-turut lc50 yaitu 283,11 ppm; 109,32 ppm dan 10,53 ppm. ekstrak metanol rumput laut gracilaria verrucosa hasil partisi dianalisis menggunakan gas chromatography mass spectrometry (gc-ms) pada menghasilkan 27 senyawa dengan senyawa konsentrasi tertinggi yaitu hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester dengan konsentrasi yaitu 47.09%. analisis gc-ms terhadap ekstrak etil asetat dan n-heksana rumput laut gracilaria verrucosa hasil partisi menghasilkan 27 dan 43 senyawa dengan senyawa konsentrasi tertinggi yaitu n-hexadecanoic acid 23.28% dan 33,89%. hasil studi in silico molecular docking menunjukkan senyawa 6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydro benzofuran-2(4h)-one yang berasal dari ekstrak etil asetat memiliki nilai energi ikatan paling besar terhadap enzim chk1 (-6,8 kkal/mol) dan enzim sod1 (-6,1 kkal/mol). kesimpulannya, hasil data aktivitas biologis menunjukkan bahwa ekstrak etil asetat dari rumput laut gracilaria verrucosa memiliki potensi sebagai antioksidan dan menimbulkan toksisitas. kata kunci : rumput laut gracilaria verrucosa., ekstrak metanol, ekstrak etil asetat, ekstrak n-heksana, antioksidan, toksisitas, dpph, bslt dan molecular docking.

The antioxidant activity and toxicity of the seaweed Gracilaria verrucosa extract have been investigated. This study aims to determine the antioxidant activity, toxicity, and chemical components of the methanol, ethyl acetate, and n-hexane extracts of Gracilaria verrucosa. Additionally, it seeks to determine the Gibbs free energy (ΔG) of the main compounds in each extract through in silico molecular docking studies. The seaweed Gracilaria verrucosa was macerated using methanol as the solvent.The methanol extract was partitioned successively with n-hexane and ethyl acetate, resulting in n-hexane and ethyl acetate extracts. Each extract was tested for its antioxidant activity and toxicity using the 1-diphenyl-2-picrylhydrazyl (DPPH) and Brine Shrimp Lethality Test (BSLT) methods. The results of the antioxidant activity test of the methanol, ethyl acetate, and n-hexane extracts yielded IC50 values of 280.82 ppm, 137.90 ppm, and 314.51 ppm, respectively. The results of the toxicity activity test of the methanol, ethyl acetate, and n-hexane extracts yielded LC50 values of 283.11 ppm, 109.32 ppm, and 10.53 ppm, respectively. The methanol extract of Gracilaria verrucosa seaweed was analyzed using Gas Chromatography Mass Spectrometry (GC-MS) and resulted in the identification of 27 compounds, with the highest concentration compound being Hexadecanoic acid, 2-hydroxy-1-(hydroxy methyl)ethyl ester, at a concentration of 47.09%. GC-MS analysis of the ethyl acetate and n-hexane extracts of Gracilaria verrucosa seaweed yielded 27 and 43 compounds, respectively, with the highest concentration compounds being n-Hexadecanoic acid at 23.28% and 33.89%, respectively. In silico molecular docking study results showed that the compound 6-Hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one from the ethyl acetate extract had the highest binding energy values to the Chk1 enzyme (-6.8 Kcal/mol) and SOD1 enzyme (-6.1 Kcal/mol). In conclusion, the results of the biological activity data indicate that the ethyl acetate extract of Gracilaria verrucosa seaweed has the potential as an antioxidant and does induce toxicity. Keywords : Gracilaria verrucosa seaweed., methanol extracts, ethyl acetate extracts, n-hexane extracts, antioxidant, toxicity, DPPH, BSLT and molecular docking.