Electronic Theses and Dissertation

abstrak jaringan ikat kolagen merupakan jaringan ikat yang terdapat pada pembungkus otot yaitu endomisium, perimisium dan epimisium yang didominasi oleh jaringan ikat kolagen tipe i dan iii. penelitian ini bertujuan untuk mengetahui distribusi jaringan ikat kolagen tipe i dan iii serta ekspresi kedua tipe jaringan ikat tersebut pada otot gluteobiceps sapi aceh. sampel penelitian berupa otot gluteobiceps yang berasal dari 3 ekor sapi aceh jantan dewasa dengan bobot badan berkisar 250-300 kg dan memiliki nilai body condition score (bcs) 3. sampel diproses menjadi preparat histologi dan diwarnai dengan pewarnaan imunohistokimia (ihk). distribusi jaringan ikat kolagen tipe i dan iii dianalisis secara deskriptif, sedangkan ekspresi jaringan ikat kolagen tipe i dan iii dinilai dengan metode intensity score (is). data is dianalisis dengan uji mann whitney u test. hasil penelitian memperlihatkan bahwa jaringan ikat kolagen tipe i dan iii terdistribusi pada lapisan endomisium dan perimisium. rata-rata nilai is jaringan ikat kolagen tipe iii lebih tinggi secara signifikan (p

ABSTRACT Collagen connective tissue is connective tissue found in muscle sheaths, namely endomysium, perimysium and epimysium which is dominated by type I and III collagen connective tissue. The aims of study was to determine the distribution of type I and III collagen connective tissue and the expression of these two types of connective tissue in the gluteobiceps muscle of Aceh cattle. The research samples were gluteobiceps muscles from 3 adult male Aceh cattle with body weights ranging from 250-300 kg and had a body condition score (BCS) of 3. The samples were processed into histology preparations and stained with immunohistochemical staining (IHC). The distribution of type I and III collagen connective tissue was analyzed descriptively, while the expression of type I and III collagen connective tissue was assessed using the intensity score (IS) method. IS data were analyzed using the Mann Whitney U Test. The results of study showed that collagen connective tissue types I and III were distributed in the endomysium and perimysium layers. The average IS value of type III collagen connective tissue was significantly higher (p