Electronic Theses and Dissertation

Daun bandotan merupakan salah satu tanaman obat yang banyak dimanfaatkan dalam mengobati berbagai jenis penyakit karena mengandung senyawa fitokimia yang dapat menghambat pertumbuhan bakteri seperti alkaloid, flavonoid, tanin, saponin dan steroid. penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol daun bandotan terhadap pertumbuhan bakteri vibrio sp. isolat ikan lele dumbo. pelaksanaan penelitian ini menggunakan metode kirby bauer disk diffusion yang diawali dengan peremajan stok isolat bakteri pada media nutrion broth, reidentifikasi dengan media thiosulfate citrate bile salt sucrose agar dan pewarnaan gram. selanjutnya dilakukan uji daya hambat menggunakan media mueller hinton agar. data hasil penelitian dianalisis secara statistik menggunkan metode one way anova dengan rancangan acak lengkap (ral) dan dilanjutkan dengan uji duncan. studi ini terdiri dari 5 perlakuan yaitu ekstrak etanol daun bandotan konsentrasi 25% (p1), 50% (p2), dan 75% (p3), siprofloksasin sebagai kontrol positif dan cmc 1% sebagai kontrol negatif. hasil penelitian menunjukkan diameter zona hambat yang terbentuk pada konsentrasi 25% (23,30mm ± 0,15), konsentrasi 50% (24,29mm ± 0,17), dan konsentrasi 75% (25,29mm ± 0,16). secara analisis statistik semua perlakuan dan kontrol menunjukkan pengaruh nyata (p

Bandotan leaves are one of the medicinal plants widely utilized in treating various diseases due to their containing phytochemical compounds that can inhibit bacterial growth such as alkaloids, flavonoids, tannins, saponins, and steroids. This study research aims to determine the activity of ethanol extract from bandotan leaves on the growth of Vibrio sp. bacteria isolated from dumbo catfish. The research was conducted using the Kirby Bauer disk diffusion method, which began with the isolation of bacterial stocks on Nutrion Broth, reidentification using Thiosulfate Citrate Bile Salt Sucrose Agar, and Gram staining. Furthermore, the inhibitory activity was tested using Mueller Hinton Agar. The research data were analyzed statistically using the One Way ANOVA method with a Completely Randomized Design (CRD) and followed by Duncan's test. This study constructed of consisting of 5 treatments: ethanol extract of bandotan leaves at concentrations of 25% (P1), 50% (P2), and 75% (P3), ciprofloxacin as positive control, and 1% CMC as negative control. The results showed the diameter of inhibition zones formed at concentrations of 25% (23.30mm ± 0.15), 50% (24.29mm ± 0.17), and 75% (25.29mm ± 0.16). Statistically, all treatments and controls showed a significant effect (P