Electronic Theses and Dissertation

Penelitian sebelumnya telah berhasil mengisolasi 16 isolat bakteri tanah dari tempat pemrosesan akhir (tpa) gampong jawa, banda aceh. penelitian ini bertujuan untuk mengetahui isolat bakteri yang dapat menghasilkan aktivitas selulase, mengkarakterisasi sifat biokimia serta mengetahui identifikasi gen 16s rrna isolat bakteri selulolitik. isolat yang telah diremajakan diuji potensi selulolitiknya berdasarkan area zona bening yang dihasilkan pada media carboxymethyl cellulose (cmc). isolat dengan indeks selulolitik terbaik selanjutnya diuji aktivitas selulolitiknya berdasarkan waktu fermentasi yaitu 18 jam, 22 jam, dan 24 jam serta variasi suhu yang digunakan adalah 35°c, 37°c, dan 40°c. uji biokimia bakteri selulolitik yang dilakukan meliputi uji methyl red-voges proskauer (mr-vp), uji triple sugar iron agar (tsia), uji simmons citrate (sc), uji katalase, uji urease, uji motilitas dan uji indol. identifikasi molekuler gen 16s rrna dilakukan untuk mengetahui kekerabatan isolat bakteri dengan organisme lain melalui pohon filogenetik. hasil penelitian menunjukkan bahwa isolat yang berpotensi menghasilkan selulase terbaik adalah isolat btpa16 dengan indeks selulolitik sebesar 2,6 dan nilai aktivitas selulolitik optimum pada suhu 37°c dan waktu inkubasi 22 jam sebesar 9,33 u/ml. berdasarkan hasil uji biokimia diduga isolat btpa16 termasuk ke dalam genus achromobacter. hasil identifikasi molekuler dengan gen 16s rrna isolat btpa16 (1402 bp) memiliki kemiripan tertinggi dengan achromobacter sp. strain mrhb1-162 sebesar 99,93%. berdasarkan rekonstruksi filogenetik isolat btpa16 memiliki kekerabatan dengan achromobacter sp. strain r2a23 dengan nilai bootstrap 81%. sehingga dapat disimpulkan bahwa isolat dengan aktivitas selulolitik terbesar yaitu btpa16 merupakan spesies dari achromobacter sp.

Previous research has succeeded in isolating 16 soil bacterial isolates from the Gampong Jawa Final Processing Site (TPA), Banda Aceh. This research aims to determine bacterial isolates that can produce cellulase activity, characterize biochemical properties and identify the 16S rRNA gene of cellulolytic bacterial isolates. The rejuvenated isolates were tested for their cellulolytic potential based on the clear zone area produced on Carboxymethyl Cellulose (CMC) media. The isolate with the best cellulolytic index was then tested for its cellulolytic activity based on the fermentation time, namely 18 hours, 22 hours and 24 hours and the temperature variations used were 35°C, 37°C and 40°C. Biochemical tests for cellulolytic bacteria carried out include the Methyl Red-Voges Proskauer (MR-VP) test, Triple Sugar Iron Agar (TSIA) test, Simmons Citrate (SC) test, catalase test, urease test, motility test and indole test. Molecular identification of the 16S rRNA gene was carried out to determine the relationship of bacterial isolates to other organisms using a phylogenetic tree. The research results showed that the isolate that had the potential to produce the best cellulase was the BTPA16 isolate with a cellulolytic index of 2.6 and an optimum cellulolytic activity value at a temperature of 37°C and an incubation time of 22 hours of 9.33 U/mL. Based on the results of biochemical tests, it is suspected that the BTPA16 isolate belongs to the Achromobacter genus. The results of molecular identification with the 16S rRNA gene of isolate BTPA16 (1402 bp) had the highest similarity to Achromobacter sp. strain MRHB1-162 was 99.93%. Based on phylogenetic reconstruction, isolate BTPA16 is related to Achromobacter sp. strain R2A23 with a bootstrap value of 81%. So it can be concluded that the isolate with the greatest cellulolytic activity, namely BTPA16, is a species of Achromobacter sp.