Electronic Theses and Dissertation

. salah satu tanaman yang menghasilkan minyak atsiri di indonesia yaitu nilam yang terkenal karena kemampuannya dalam menghasilkan minyak nilam. perolehan benih tanaman nilam dan stek tersebut ditanam pada media polibag. perolehan benih tanaman nilam belum optimal disebabkan oleh lemahnya perbanyakan vegetatif tradisional, yaitu lamanya waktu yang dibutuhkan untuk menghasilkan benih baru. kultur jaringan atau metode perbanyakan tanaman in vitro merupakan cara untuk mengatasi masalah kelangkaan pasokan benih. penelitian ini menggunakan eksplan pucuk nilam varietas tapak tuan. tujuan dilakukannya penelitian ini adalah untuk mengetahui pengaruh konsentrasi indole acetic acid (iaa) dan benzyl amino purine (bap) serta interaksi antara kedua kombinasi konsentrasi terhadap induksi pucuk tanaman nilam varietas tapak tuan secara in vitro yang dilaksanakan pada bulan januari sampai juni 2023 di laboratorium kultur jaringan tanaman jurusan agroteknologi fakultas pertanian universitas syiah kuala banda aceh. adapun yang diamati yaitu persentase hidup eksplan, jumlah tunas, jumlah daun dan jumlah akar. hasil penelitian menunjukkan bahwa persentase hidup eksplan nilam 100% dijumpai pada semua kombinasi konsentrasi. jumlah tunas yaitu 8,2 tunas dijumpai pada kombinasi konsentrasi iaa 0,25 ml l-1 + bap 0,5 ml l-1. jumlah daun yaitu 7,8 daun dijumpai pada kombinasi konsentrasi iaa 0,5 ml l-1 + bap 0,25 ml l-1. jumlah akar yaitu 4,2 akar dijumpai pada kombinasi konsentrasi iaa 0,5 ml l-1 + bap 0 ml l-1.

One of the plants that produces essential oil in Indonesia is patchouli which is famous for its ability to produce patchouli oil.Patchouli plant seeds were obtained and the cuttings were planted in polybag media. The production of patchouli seeds is not optimal due to the weakness of traditional vegetative propagation, namely the long time required to produce new seeds. Tissue culture or in vitro plant propagation methods are a way to overcome the problem of scarcity of seed supply. This research used patchouli shoot explants of the Tapak Tuan variety. The aim of this research was to determine the effect of concentrations of Indole Acetic Acid (IAA) and Benzyl Amino Purine (BAP) as well as the interaction between the two concentration combinations on shoot induction of the Tapak Tuan variety patchouli plants in vitro which was carried out from January to June 2023 at Laboratorium Kultur Jaringan Tanaman Jurusan Agroteknologi Fakultas Pertanian Universitas Syiah Kuala Banda Aceh .What was observed was the percentage of live explants, number of shoots, number of leaves and number of roots. The results showed that the survival percentage of patchouli explants was 100% found in all concentration combinations. The number of shoots, namely 8.2 shoots, was found in the combination concentration of IAA 0.25 ml L-1 + BAP 0.5 ml L-1. The number of leaves, namely 7.8 leaves, was found in the combination concentration of IAA 0.5 ml L-1 + BAP 0.25 ml L-1. The number of roots, namely 4.2 roots, was found in the combination concentration of IAA 0.5 ml L-1 + BAP 0 ml L-1.