Electronic Theses and Dissertation

Stress oksidatif memiliki peran utama terhadap progresi komplikasi dari diabetes melitus yang berpengaruh pada system kardiovaskular. disfungsi endotel yang dipengarahui oleh nitrit oksida (no) dan superoksida dismustase (sod) memiliki peran penting terhadap proses tersebut. pandemi covid-19 suplemen seperti asam askorbat dan kalsitriol banyak digunakan dan memiliki efek yang menjanjikan. untuk saat ini belum ada mekanisme yang jelas untuk membuktikan peran asam askorbat dan kalsitriol dalam perbaikan disfungsi endotel pada patogenesis aterosklerosis dengan kondisi dm tipe 2. tujuan penelitian ini adalah untuk menilai dan membandingkan efek asam askorbat dan kalsitriol terhadap kadar no dan sod. studi ini dilakukan secara in vivo melibatkan 4 ekor tikus putih rattus novergicus strain wistar jantan yang berumur 4 minggu, sehat dengan berat badan 50-100 gr yang dibagi menjadi 4 kelompok dengan jumlah yang sama yakni; kelompok kontrol/ normal (n), kelompok dislipidemia (dl) dan dm tipe 2 (dm), kelompok dl serta dm yang diberikan asam askorbat (dmc) dan kelompok dl serta dm yang diberikan kalsitriol (dmd). diet diberikan selama 90 hari dan pemberian intervensi dengan asam askorbat dan kalsitriol selama 30 hari. asam askorbat dan kalsitriol memilik efek meningkatkan kadar no dan sod (p

Oxidative stress plays a crucial role in the progression of complications arising from diabetes, affecting cardiovascular. Endothelial dysfunction, brought about by factors like nitric oxide (NO) and superoxide dismutase (SOD), contributes significantly to this process. In the context of the COVID-19 pandemic, supplements like ascorbic acid and calcitriol have gained prominence. Despite several studies elaborating on their ability to reduce oxidative stress, the exact mechanisms is still uncertain. The purpose of this study was exploring the comparative impact of ascorbic acid also calcitriol on nitric oxide (NO) and superoxide dismutase (SOD) levels. These agents serve as protective factors against free radicals, which had major effect in the first stage of atherosclerosis and diabetes-related issues. The study utilized an experimental in vivo approach, involving 24 male Wistar rats of the Rattus novergicus strain. The rats were categorized into four groups: N (received a standard diet, acting as the control), DM (administered an atherogenic diet and streptozocin to induce diabetes), DMC (given an atherogenic diet, streptozocin, and ascorbic acid), and DMD (administered an atherogenic diet, streptozocin, and calcitriol). The intervention was carried out over a span of 90 days, with a 30-day intervention period. Both ascorbic acid and calcitriol exhibited significant effectiveness (p < 0.05) in elevating levels of NO and SOD. Calcitriol outperformed ascorbic acid in increasing NO and SOD levels (p < 0.05). In a rat model of type 2 diabetes with atherosclerosis, both ascorbic acid and calcitriol demonstrated an ability to enhance NO and SOD levels. Notably, calcitriol exhibited a greater capacity to increase NO and SOD levels rather than ascorbic acid. Keywords: Ascorbic acid, Calcitriol, SOD, NO, Type 2 diabetes melitus, Endothelial dysfunction