Electronic Theses and Dissertation

Fatigue merupakan ketidakmampuan tubuh dalam merespon suatu stimulus yang disebabkan oleh laktat sehinga menganggu metabolisme tubuh. kondisi ini dapat dicegah dengan pemberian powder supplement dan ergogenik lainnya (abbiss & laursen, 2005). penelitian powder supplement terus dikembangkan diantaranya dari daun moringa oleifera dan carica papaya yang memiliki aktivitas antifatigue. lokasi tumbuh tanaman berpengaruh pada metabolit sekunder yang dihasilkan. penelitian tahap i menunjukkan bahwa tekstur partikel tanah di lambhuk, kajhu, dan ie seum secara berurutan adalah lempung, lempung berpasir, dan lempung berdebu. penelitian tahap ii memperoleh taksonomi tanaman papaya yaitu c. papaya dan tanaman kelor yaitu moringa oleifera. hasil ph tanah daerah kajhu, ie seum, lambhuk masing-masing 6.6, 5.1, 5.9. dan suhu tanah 38.5oc, 33.9 oc, dan 32.4 oc. penelitian tahap iii, metabolit sekunder daun c. papaya di daerah kajhu dan lambhuk yaitu alkaloid, steroid, saponin, flavonoid, dan fenolik. sampel daun dari ie seum tidak memiliki saponin. daun m. oleifera dari ketiga lokasi mengandung alkaloid, steroid, saponin, flavonoid, fenolik, dan tannin. penelitian tahap iv diperoleh hasil uji gc-ms daun c. papaya dari kajhu, lambhuk dan ie seum masing-masing 24, 23, 17 senyawa dan untuk m. oleifera masing-masing 28, 24, dan 27 senyawa. senyawa yang mengandung efek antifatigue adalah neophytadiene, hexadecanoic acid; phytol; 9,12,15- octadecatrienoic acid; linolenic acid; squalene; gamma.-tocopherol; vitamin e; carpaine; methyl commate b dan 5-isopropyl-6-methyl-hepta-3,5-dien-2-ol. penelitian tahap v menganalisa efek powder supplement terhadap fatigue tikus wistar (kontrol negatif (k1), c. papaya 200 mg (k2), m. oleifera 200 mg (k3), c. papaya + m. oleifera 200 mg (k4), c. papaya 400 mg (k5), m. oleifera 400 mg (k6) dan c. papaya + m. oleifera 400 mg (k7)). k5 memiliki antifatigue tertinggi (35.65 ± 22.25). histologi serat otot sama untuk semua kelompok dan angiogenesis memiliki nilai bermakna pada k2, k3, k5, dan k7.

Fatigue is the body's inability to respond to a stimulus caused by lactate, thereby disrupting the body's metabolism. This condition can be prevented by giving powder supplements and other ergogenic (Abbiss & Laursen, 2005). Powder supplement research continues to be developed, including those from the leaves of Moringa oleifera and Carica papaya which have antifatigue activity. The location of plant growth affects the secondary metabolites produced. Phase I research showed that the texture of soil particles in Lambhuk, Kaju, and Ie Seum were loam, sandy loam, and dusty loam, respectively. The research II obtained the taxonomy of papaya namely C. papaya and kelor namely Moringa oleifera. The soil pH results for the Kajhu, Ie Seum, Lambhuk areas were 6.6, 5.1, 5.9, respectively. and soil temperature 38.5oC, 33.9oC and 32.4oC. Phase III of the study, the secondary metabolites of C. papaya leaves in the Kajhu and Lambhuk areas were alkaloids, steroids, saponins, flavonoids, and phenolics. Leaf samples from Ie Seum did not have saponins. M. oleifera leaves from the three locations contained alkaloids, steroids, saponins, flavonoids, phenolics, and tannins. Phase IV research obtained GC-MS test results for C. papaya leaves from Kajhu, Lambhuk and Ie Seum respectively 24, 23, 17 compounds and for M. oleifera each 28, 24, and 27 compounds. Compounds that contain antifatigue effects are Neophytadiene, Hexadecanoic acid; Phytol; 9,12,15- octadecatrienoic acid; Linolenic acid; Squalene; Gamma.-Tocopherol; Vitamin E; Carpaine; Methyl Commate B dan 5-Isopropyl-6-methyl-hepta-3,5-dien-2-ol. Stage V study analyzed the effect of powder supplement on fatigue of Wistar rats (negative control (K1), C. papaya 200 mg (K2), M. oleifera 200 mg (K3), C. papaya + M. oleifera 200 mg (K4), C. . papaya 400 mg (K5), M. oleifera 400 mg (K6) and C. papaya + M. oleifera 400 mg (K7)). K5 had the highest anti-fatigue (35.65 ± 22.25). Muscle fiber histology was the same for all groups and angiogenesis was of significant value at K2, K3, K5, and K7.