Electronic Theses and Dissertation

Biosintesis nanopartikel tembaga (cu) menggunakan ekstrak metanol akar srikaya (annona squamosa) dengan prekursor cuso4 serta uji antimikroba dan antikanker telah berhasil dilakukan. pengujian total phenolic content (tpc) ekstrak metanol akar srikaya diperoleh sebesar 182 mg gae/g dan total flavonoid content (tfc) ekstrak metanol akar srikaya diperoleh sebesar 4,1 mg qe/g. morfologi dan struktur nanopartikel cu hasil sintesis dikarakterisasi dengan spektrofotometer uv-vis, fourier transform infrared (ftir), x-ray diffraction (xrd), scanning electron microscope-energy dispersive x-ray (sem-edx), dan transmission electron microscopy (tem). hasil spektroskopi uv-vis mengungkapkan keberadaan nanopartikel cu. spektrum ftir mengidentifikasi gugus fungsi yang terlibat dalam sintesis nanopartikel cu. difraktogram xrd menunjukkan bahwa produk sintesis yang terbentuk adalah cu(0), cu2o, cuo, dan cuo2. nanopartikel cu yang disintesis teraglomerasi seperti yang dikonfirmasi oleh sem-edx. mikrograf tem dapat dilihat bahwa distribusi ukuran partikel nanopartikel cu memiliki keseragaman yang tinggi dengan ukuran 2-10 nm. nanopartikel yang disintesis memiliki aktivitas antimikroba terhadap bakteri staphylococcus aureus, bakteri escherichia coli dan jamur candida albicans. nanopartikel yang disintesis juga menunjukkan kategori sitotoksisitas kuat terhadap sel kanker payudara manusia (mcf-7). kata kunci: akar srikaya, nanopartikel tembaga, aktivitas antimikroba, aktivitas antikanker

Biosynthesis of copper (Cu) nanoparticles using methanol extract of the root of srikaya (Annona squamosa) with CuSO4.5H2O precursor and antimicrobial and anticancer tests have been successfully conducted. Total Phenolic Content (TPC) of methanol extract of the root of srikaya was 182 mg GAE/g and Total Flavonoid Content (TFC) of methanol extract of the root of srikaya was 4.1 mg QE/g. The morphology and structure of the synthesized Cu nanoparticles were characterized by UV-Vis spectrophotometer, Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscope-Energy Dispersive X-Ray (SEM-EDX), and Transmission Electron Microscopy (TEM). UV-Vis spectroscopy results showed the presence of Cu nanoparticles. FTIR spectra identified the functional groups involved in the synthesis of Cu nanoparticles. XRD diffractograms showed that the synthesis products formed were Cu(0), Cu2O, CuO, and CuO2. The synthesized Cu nanoparticles were agglomerated as confirmed by SEM-EDX. From TEM micrographs, it can be seen that the particle size distribution of Cu nanoparticles has high uniformity with a size of 2-10 nm. The synthesized nanoparticles have antimicrobial activity against Staphylococcus aureus bacteria, Escherichia coli bacteria and Candida albicans fungus. The synthesized nanoparticles also showed strong cytotoxicity against human breast cancer cells (MCF-7). Keywords: Root srikaya, copper nanoparticles, antimicrobial activity, anticancer activity