Electronic Theses and Dissertation

Transplantasi ovarium sapi aceh pada uterus kelinci new zealand white (nzw) sebagai host transplantasi diduga dapat menimbulkan stres. dampak stres ini akan menyebabkan perubahan histopatologis ovarium. dampak ini dapat dikurangi dengan pemberian ekstrak tanaman sarang semut (myrmecodia pendans) yang bersifat sebagai antioksidan. penelitian ini bertujuan mengetahui peningkatan jumlah folikel utuh dan gambaran histopatologis ovarium sapi aceh setelah ditransplantasi di dalam uterus kelinci bunting semu yang diberikan ekstrak tanaman sarang semut. dalam penelitian ini digunakan sembilan ekor kelinci betina nzw bunting semu, berumur 3-5 tahun dan bobot badan 1,5-3,0 kg. kelinci dikelompokkan dalam tiga kelompok perlakuan (n=3), yaitu k0, k1, dan k2. seluruh kelinci diinduksi bunting dengan injeksi 100 iu pmsg dan diikuti tiga hari kemudian dengan injeksi 75 iu hcg secara intramuskulus. transplantasi ovarium dilakukan pada hari ke-8 (hari ke-0 adalah hari ketika injeksi hcg). perlakuan pada semua kelompok dilakukan setiap pagi dan sore hari mulai pelaksanaan transplantasi ovarium sampai ovarium dikoleksi yang terdiri atas pemberian nacl fisiologis (k0), ekstrak tanaman sarang semut dengan dosis 200 mg per oral (k1), dan kombinasi cyclosporine 2,5 mg/kg secara intramuskulus dan azathioprine dosis 5 mg/kg bobot badan per oral (k2). ovarium dikoleksi lima hari setelah transplantasi lalu diproses secara histoteknik untuk pengamatan histopatologis ovarium. hasil pengamatan ditemukan beberapa perubahan histopatologis ovarium meliputi struktur jaringan yang kurang padat, lapisan sel teka dan sel granulosa tidak utuh, sel granulosa terlepas dari lapisan dan masuk ke antrum, zona pelusida tidak utuh, dan adanya vakuola antara lapisan sel granulosa dan sel teka. jumlah folikel utuh pada k1; k2; dan k3 pada folikel primordial (6,00±1,00; 22,33±3,05; dan 22,00±23,43), folikel primer (5,67±0,58; 16,33±5,51; dan 18,00±16,64), folikel sekunder (2,67±1,53; 6,00±4,36; dan 9,67±10,79), dan folikel tersier (1,00±1,00; 1,00±1,00; dan 4,33±3,21). meskipun menunjukkan perbedaan yang tidak signifikan (p>0,05), jumlah folikel primordial dan folikel primer pada k1 dan k2 cenderung lebih banyak dibandingkan k0 sedangkan pada folikel sekunder dan tersier jumlahnya relatif sama. disimpulkan bahwa pemberian ekstrak sarang semut selama periode transplantasi di dalam uterus kelinci nzw bunting semu cenderung meningkatkan jumlah folikel utuh dan menurunkan histopatologis ovarium sapi aceh

Ovary transplantation of aceh cattle to the uterus of New Zealand White (NZW) rabbits as a transplant host is thought to cause stress. The impact of this stress will cause histopathological change of ovary. This impact can be reduced by giving ant nest plant extract (Myrmecodia pendans) which acts as an antioxidant. This study aims to determine the increase in the number of intact follicles and the histopathological appearance of the ovaries of aceh cattle after being transplanted into the uterus of pseudo-pregnant rabbits given ant nest plant extract. In this study nine pseudopregnant NZW female rabbits, aged 3-5 years and 1.5-3.0 kg body weight were used. Rabbits were grouped into three treatment groups (n=3), namely K0, K1, and K2. All rabbits were induced pregnant with an injection of 100 IU of PMSG and followed three days later by an intramuscular injection of 75 IU of hCG. Ovarian transplantation was performed on day 8 (day 0 was the day of hCG injection). Treatment in all groups was carried out every morning and evening from the implementation of the ovarian transplantation until the ovaries were collected consisting of administration of physiological NaCl (K0), ant nest plant extract at a dose of 200 mg orally (K1), and a combination of cyclosporine 2.5 mg/kg intramuscularly and azathioprine dose of 5 mg/kg body weight orally (K2). The ovaries were collected five days after transplantation and then processed for histopathological observation of the ovaries. The results of observations found several histopathological changes of aceh cattle ovaries including less dense tissue structure, incomplete layers of theca cells and granulosa cells, granulosa cells detached from the layers and entered the antrum, the zona pellucida was not intact, and the presence of vacuoles between the granulosa cell layers and theca cells. Number of intact follicles on K1; K2; and K3 in primordial follicles (6.00 ± 1.00; 22.33 ± 3.05; and 22.00 ± 23.43), primary follicles (5.67 ± 0.58; 16.33 ± 5.51 ; and 18.00 ± 16.64), secondary follicles (2.67 ± 1.53; 6.00 ± 4.36; and 9.67 ± 10.79), and tertiary follicles (1.00 ± 1, 00; 1.00 ± 1.00; and 4.33 ± 3.21). Although the difference was not significant (P>0.05), the number of primordial and primary follicles in K1 and K2 tended to be more than K0, while the number of secondary and tertiary follicles was relatively the same. It was concluded that administration of ant nest extract during the transplantation period in the uterus of pseudo-pregnant NZW rabbits tended to increase the number of intact follicles and decrease the histopathological of the ovaries of aceh cattle.