Electronic Theses and Dissertation

Daun senggani merupakan tanaman semak belukar yang berkhasiat obat secara empiris dan sebagai antibakteri secara ilmiah, karena memiliki kandungan fitokimia berupa flavonoid, alkaloid, saponin, tanin, dan steroid. penelitian ini bertujuan untuk mengetahui efektivitas ekstrak etanol daun senggani (melastoma candidum) terhadap daya hambat pertumbuhan bakteri vibrio parahaemolyticus secara in vitro. perlakuan ini dilakukan dengan metode kirby-bauer terhadap bakteri atcc yang sudah dire-identifikasi dan menggunakan ekstrak etanol daun senggani konsentrasi 25%, 50%, dan 75%, dengan kloramfenikol sebagai kontrol positif dan cmc 1% sebagai kontrol negatif. masing-masing disk perlakuan dan kontrol ditempelkan pada permukaan media mha yang sudah diswab ratakan bakteri, kemudian diinkubasi selama 24 jam pada suhu 37°c. diameter zona hambat yang terbentuk di sekitar disk diukur dengan menggunakan jangka sorong dan dinyatakan dalam satuan millimeter (mm). hasil pengamatan menunjukkan diameter zona hambat konsentrasi 25% sebesar 8.32 ± 0.120 mm, 50% sebesar 8.59 ± 0.286, dan 75% sebesar 8.74 ± 0.205 mm, pada kontrol positif (kloramfenikol) sebesar 22.35 ± 0.648 dan kontrol negatif cmc 1% tidak terbentuk zona hambat. hasil penelitian dapat disimpulkan bahwa semua konsentrasi ekstrak etanol daun senggani memiliki aktivitas daya hambat terhadap pertumbuhan bakteri vibrio parahaemolyticus dalam kategori lemah

Senggani leaves are a shrub plant that has medicinal properties empirically and as an antibacterial scientifically, because they contain phytochemicals such as flavonoids, alkaloids, saponins, tanins, and steroids. This research aims to determine the effectiveness of ethanol extract of senggani leaves (Melastoma candidum) on the growth inhibition of Vibrio parahaemolyticus bacteria in vitro. The treatment was carried out using the Kirby-Bauer method against ATCC bacteria that had been identified and using ethanol extract of senggani leaves at concentrations of 25%, 50% and 75%, with chloramphenicol as a positive control and 1% CMC as a negative control. Each treatment and control disk was affixed to the surface of MHA media that had been dripped with bacteria, then incubated for 24 hours at 37°C. The diameter of the inhibition zone formed around the discs was measured with a caliper and expressed in military (mm). The observation results showed the diameter of the inhibition zone on the 25% concentration was 8.32 ± 0.120 mm, 50% was 8.59 ± 0.286, and 75% was 8.74 ± 0.205 mm, on the positive control (chloramphenicol) was 22.35 ± 0.648 and the negative control 1% CMC had no inhibition zone. The results of the study it can be concluded that all concentrations of ethanol extract of senggani leaves have inhibitory activity against the growth of Vibrio parahaemolyticus bacteria in the weak category.