Electronic Theses and Dissertation

Black soldier fly (hermetia illucens) yang dikenal dengan sebutan maggot merupakan salah satu alternatif bahan pakan ternak yang memiliki kandungan protein tinggi sekitar 40-50%. pakan asal larva bsf harus aman dari kontaminan bakteri, bahan kimia, jamur, dan lainnya. umumnya identifikasi bakteri dilakukan dengan metode konvensional. namun, metode tersebut memiliki beberapa kelemahan sehingga identifikasi dilakukan dengan metode identifikasi berbasis molekuler dengan menganalisis urutan gen 16s rrna. tujuan penelitian ini untuk mengidentifikasi bakteri asal larva bsf berdasarkan analisis gen 16s rrna. sampel yang digunakan sebanyak tujuh isolat bakteri asal larva bsf. rancangan penelitian dimulai dengan tahap ekstraksi dna dilanjutkan dengan amplifikasi dna metode polymerase chain reaction (pcr), kemudian sekuensing, analisis bioinformatika, serta konstruksi pohon filogenetik. hasil penelitian identifikasi bakteri asal larva bsf menunjukkan isolat m1-4, m1-8, m1-25, m2-4, m2-6, m2-16 dan m2-34 dapat teramplifikasi dengan primer 27f dan 1429r dengan ukuran fragmen dna 1500 pb. berdasarkan hasil analisis blast.n ketujuh isolat menunjukkan persentase kemiripan dengan database di genbank rata-rata >99% dengan bakteri berturut-turut providencia vermicola strain gol12, morganella morganii subsp. morganii strain alk057, p. vermicola strain gol12, enhydrobacter sp. strain gamma-17, bacillus albus strain pja8.2, p. rettgeri strain bml2496 dan p. rettgeri strain 2ajs. hasil analisis konstruksi pohon filogenetik menunjukkan hubungan kekerabatan yang terbagi menjadi empat klaster kekerabatan yaitu klaster genus providencia, klaster genus morganella, klaster genus enhydrobacter dan klaster genus bacillus serta terpisah dari klaster outgroup genus streptomyces. dapat disimpulkan bahwa dari tujuh isolat terdapat empat jenis bakteri yang diidentifikasi sebagai bakteri patogen dan patogen oportunistik.

Black Soldier Fly (Hermetia illucens) known as maggot is an alternative animal feed ingredient that has a high protein content of around 40-50%. The feed from BSF larvae must be safe from bacterial, chemical, fungal and other contaminants. Generally, the identification of bacteria is done by conventional methods. However, the method has several disadvantages so that identification is carried out with molecular-based identification methods by analyzing the 16S rRNA gene sequence. The purpose of this study was to identify the bacterial origin of BSF larvae based on the analysis of the 16S rRNA gene. The samples used were seven isolates of bacteria from BSF larvae. The research design began with the DNA extraction stage followed by DNA amplification of the Polymerase Chain Reaction (PCR) method, then sequencing, bioinformatics analysis, and phylogenetic tree construction. The results of the identification of bacteria from BSF larvae showed that isolates of M1-4, M1-8, M1-25, M2-4, M2-6, M2-16 and M2-34 can be amplified with primers of 27f and 1429r with a DNA fragment size of 1500 pb. Based on the results of BLAST.N analysis seven isolates showed a percentage of similarity to the database in GenBank averaging >99% with successive bacteria Providencia vermicola strain Gol12, Morganella morganii subsp. Morganii strain ALK057, P. vermicola strain Gol12, Enhydrobacter sp. strain Gamma-17, Bacillus albus strain PJA8.2, P. rettgeri strain BML2496 and P. rettgeri strain G0519 . The results of phylogenetic tree construction analysis show kinship relationships that are divided into four kinship clusters, namely the Genus Providencia cluster, the Genus Morganella cluster, the Genus Enhydrobacter cluster and the Genus Bacillus cluster and separate from the outgroup cluster of the Genus Streptomyces. It can be concluded that of the seven isolates there are four types of bacteria identified as pathogenic bacteria and opportunistic pathogens.