Electronic Theses and Dissertation

Infeksi bakteri telah menjadi perhatian utama pada sebagian usaha peternakan sapi. keberadaan bakteri pada preputium sapi berperan penting dalam transfer penyakit serius pada musim kawin dan saat penampungan semen segar. penelitian ini bertujuan untuk mengetahui keragaman bakteri pada preputium sapi aceh dengan melakukan isolasi, karakterisasi, identifikasi dan manifestasi bakteri pada preputium sapi aceh dengan pendekatan analisis gen 16s rrna. sebanyak 75 sampel swab preputium yang diperoleh dari balai pembibitan ternak unggul hijauan pakan ternak (bptu-hpt) indrapuri- aceh besar. sampel diisolasi menggunakan metode standar mikrobiologi sederhana dan dianalisis secara biologi molekuler. hasil penelitian menggunakan metode kultur teridentifikasi 6 genus bakteri diantaranya; staphylococcus dan bacillus sebagai genus bakteri gram positif serta klebsiella, acinetobacter, comamonas dan escherichia sebagai genus bakteri gram negatif. hasil secara biologi molekur dengan menggunakan gen 16s rrna menunjukkan urutan filogentik terhadap 6 spesies bakteri diantaranya bakteri staphylococcus pasteuri strain atcc 51129; bacillus cereus strain jcm 2152 16s; klebsiella quasipneumoniae subsp. similipneumoniae strain 07a044; acinetobacter soli strain b1 dan bakteri comamonas kerstersii strain lmg 3475, serta bakteri escherichia fergusonii strain atcc 35469. dapat disimpulkan bahwa pada preputium sapi aceh yang sehat secara klinis menujukkan adanya 6 spesies bakteri bersifat patogen oportunistik yang berpotensi menganggu sistem reproduksi dan menyebabkan infertil pada ternak. keberadaan bakteri pada preputium diduga berasal dari kontaminasi bakteri lingkungan, sehingga diperlukan adanya pelaksanaan survei rutin sebagai upaya pencegahan kontaminasi bakteri, serta diperlukan juga upaya peningkatan kesadaran pekerja terhadap standar kebersihan di lingkungan peternakan.

Bacterial infection has become a major concern in some cattle farm businesses. The presence of bacteria in the prepuce of bulls plays an important role in the transfer of severe diseases during mating season and fresh semen collections. This study aims to determine the diversity of bacteria in the prepuce of Aceh cattle by conducting isolation, characterization, identification, and manifestation of bacteria with the 16s rRNA gene analysis approach. A total of 75 samples of prepuce swabs were collected from the bulls in the Nursery Center for Livestock and Fodder Crops (BPTU-HPT) in Indrapuri-Aceh Besar. Samples were isolated using simple microbiological methods and analyzed using molecular biology. The results showed that the culture method could identify 2 genera of Gram-positive bacteria namely Staphylococcus and Bacillus, and 4 genera of Gram-negative bacteria namely Klebsiella, Acinetobacter, Comamonas and Escherichia. Molecular biological results using the 16S rRNA gene showed a phylogenetic sequence of 6 species of bacteria including Staphylococcus Pasteuri strain ATCC 51129; Bacillus cereus strain JCM 2152 16s; Klebsiella quasipneumoniae subsp. similipneumoniae strain 07A044; Acinetobacter Soli strain B1; Comamonas kerstersii strain LMG 3475 and Escherichia fergusonii strain ATCC 35469. It can be concluded that there are 6 species of opportunistic pathogenic bacteria that can be found in the prepuce of healthy Aceh cattle that may cause reproductive disorders and infertility in livestock. The presence of bacteria in the prepuce is thought to originate from the contamination of environmental bacteria, so it is necessary to conduct routine surveys in an effort to prevent bacterial contamination, which may lead to reproductive diseases, as well as to increase awareness of sanitary standards in the livestock environment among farm workers.