Electronic Theses and Dissertation

Progesteron merupakan salah satu jenis hormon steroid yang berpengaruh pada pola reproduksi ternak, hormon tersebut dihasilkan oleh korpus luteum dan berfungsi untuk memelihara kebuntingan pada hewan normal. saat menjelang partus hormon progesteron menurun karena dikonversi menjadi estrogen oleh bantuan 3 enzim. tujuan penelitian ini untuk mengetahui kadar hormon progesteron menjelang partus pada kuda gayo yang dipelihara secara tradisional. penelitian ini menggunakan sampel darah dilakukan di vena jugularis pada 3 ekor kuda gayo betina bunting. pengukuran hormon progesteron dilakukan dengan metode elisa (enzyme linked immunosorbent essay). pengambilan sampel darah dilakukan di vena jugularis pada kuda betina bunting. penetapan pengambilan sampel plasma darah sebanyak 3 kali dengan interval waktu 7 hari pada bulan terakhir menjelang partus, kemudian pengambilan sampel darah dilanjutkan setiap hari menjelang partus (d -1) atau sampai partus (d 0). penentuan waktu menjelang partus berdasarkan runutan ulang kebelakang pengambilan sampel terhitung dari hari saat partus (d 0). hasil pengamatan terhadap konsentrasi hormon progesteron pada 3 ekor kuda gayo betina satu bulan menjelang partus dapat diketahui bahwa terjadi penurunan kadar hormon progesteron sejak d -22 sampai dengan d -1 menjelang partus, yaitu 100,38±75,60 – 5,19±3,39 ng/ml. berdasarkan hasil penelitian ini dapat disimpulkan bahwa adanya penurunan kadar hormon progesteron pada d -1 yang dapat menjadi indikator waktu partus pada kuda gayo.

ABSTRACT Progesterone is a type of steroid hormone that affects the reproductive pattern of livestock, this hormone is produced by the corpus luteum and functions to maintain pregnancy in normal animals. Just before parturition the hormone progesterone decreases because it is converted to estrogen by the help of 3 enzymes. The purpose of this study was to determine the levels of the hormone progesterone before parturition in gayo horses that were reared traditionally. This study used blood samples taken in the jugular vein of 3 pregnant female gayo horses. Measurement of the hormone progesterone was carried out using the ELISA (enzyme linked immunosorbent essay) method. Blood sampling was performed in the jugular vein in pregnant mares. Determination of blood plasma sampling 3 times with an interval of 7 days in the last month before parturition, then blood sampling is continued every day before parturition (D -1) or until parturition (D 0). Determination of the time leading up to parturition based on backwards sampling sequences from the day of parturition (D 0). The results of observing the concentration of the hormone progesterone in 3 female Gayo horses one month before parturition showed that there was a decrease in levels of the hormone progesterone from D -22 to D -1 before parturition, namely 100.38 ± 75.60 – 5.19 ± 3 .39ng/ml. Based on the results of this study it can be concluded that there is a decrease in levels of the hormone progesterone at D -1 which can be an indicator of parturition time in gayo horses.