Electronic Theses and Dissertation

Penelitian ini bertujuan untuk mengetahui aktivitas antikanker pada sel lini t47d secara in vitro dan profil metabolit sekunder melalui analisis gas chromatography- mass spectrometry (gcms) dari bakteri endofit tapak dara staphylococcus sp. betd5. metode yang digunakan pada penelitian ini adalah metode eksperimental. parameter yang diamati pada aktivitas antikanker meliputi viabilitas sel dan nilai inhibition concentration 50% (ic50), sedangkan pada analisis gcms yaitu persentase peak area yang mempresentasikan identifikasi komponen kimia. aktivitas antikanker dilakukan menggunakan metode microtetrazolium (mtt) assay dengan konsentrasi sampel yaitu 3,125; 6.25; 12.5; 25; dan 50 ppm. profil metabolit sekunder dapat diketahui melalui analisis gcms. hasil penelitian menunjukkan bahwa aktivitas antikanker supernatan staphylococcus sp. betd5 memiliki sifat sitotoksik yang kuat dengan nilai ic50 sebesar 14,28 ppm. berdasarkan analisis gcms diperoleh tiga senyawa tertinggi yaitu cis-ocimene (24.31%), 2-beta pinene (13.98%), dan 1- phellandrene (11.67%). kata kunci: antikanker, t47d, bakteri endofit, gcms, metabolit sekunder.

This study aims to determine the anticancer activity of the T47D cell line in vitro and the profile of secondary metabolites through Gas Chromatography-Mass spectrometry (GCMS) analysis from endophytic bacteria rose periwinkle of Staphylococcus sp. BETD5. The method used in this research is an experimental method. Parameters observed in anticancer activity included cell viability and the value Inhibition Concentration 50% (IC50), while in GCMS analysis, namely the percentage of peak areas which represented the identification of chemical components. Anticancer activity was carried out using the Microtetrazolium (MTT) assay method with sample concentrations of 3.125; 6.25; 12.5; 25; and 50 ppm. Secondary metabolite profiles can be identified through GCMS analysis. The results showed that the anticancer activity of supernatant Staphylococcus sp. BETD5 has strong cytotoxic properties with an IC50 value of 14.28 ppm. Based on GCMS analysis, the three highest compounds were obtained, namely Cis-ocimene (24.31%), 2-Beta pinene (13.98%), and 1- Phellandrene (11.67%). Keywords: Anticancer, T47D, endophytic bacteria, GCMS, secondary metabolites.