Electronic Theses and Dissertation

Penelitian ini bertujuan untuk mengetahui pengaruh penambahan air kelapa dan gliserol pada penyimpanan spermatozoa ikan komet (carassius auratus) terhadap motilitas, viabilitas spermatozoa ikan komet (carassius auratus) dan derajat pembuahan telur ikan komet. pada penelitian ini menggunakan eksperimental rancangan acak lengkap (ral) dengan 4 perlakuan dan 5 ulangan yaitu p0 (sperma + 100% nacl), p1 (sperma + 50% air kelapa + 50% gliserol), p2 (sperma + 60% air kelapa + 40% gliserol) dan p3 (sperma + 70% air kelapa + 30% gliserol). hasil penelitian menunjukkan bahwa penambahan air kelapa dan gliserol pada penyimpanan sperma ikan komet berpengaruh nyata (˂0,05) terhadap nilai motilitas dan viabilitas. berdasarkan hasil penelitian dapat disimpulkan bahwa nilai motilitas dan viabilitas spermatozoa pasca kriopreservasi terbanyak pada ikan komet dalam penelitian ini terdapat pada perlakuan p2 dengan konsentrasi 60% air kelapa + 40% gliserol. kata kunci: spermatozoa, air kelapa, gliserol, motilitas, viabilitas

This study aimed to determine the effect of adding coconut water and glycerol on the storage of comet fish (Carassius auratus) spermatozoa on motility, sperm viability of comet fish (Carassius auratus) and the degree of fertilization of comet fish eggs. This study used a completely randomized experimental design (CRD) with 4 treatments and 5 replications, namely P0 (Sperm + 100% NaCl), P1 (Sperm + 50% coconut water + 50% glycerol), P2 (Sperm + 60% coconut water. + 40% glycerol) and P3 (Sperm + 70% coconut water + 30% glycerol). The results showed that the addition of coconut water and glycerol on sperm storage of comet fish had a significant effect (˂0.05) on the value of motility and viability. Based on the results of the study, it can be concluded that the highest post-cryopreservation spermatozoa motility and viability values in comet fish were found in P2 treatment with a concentration of 60% coconut water + 40% glycerol. Keywords: Spermatozoa, coconut water, glycerol, motility, viability