Electronic Theses and Dissertation

Aktinobakteri tanah berpotensi menghasilkan senyawa antimikroba. senyawa antimikroba bermanfaat dalam pengobatan penyakit infeksi yang disebabkan oleh bakteri dan cendawan. penelitian ini bertujuan untuk menguji potensi isolat aktinobakteri asal tanah ujong pancu, aceh besar, sebagai penghasil antimikroba. sebanyak sembilan isolat aktinobakteri diuji potensinya sebagai penghasil antimikroba terhadap mikroba uji staphylococcus aureus, escherichia coli, dan candida albicans melalui uji antagonis dengan metode kirby-bauer pada cakram agar dan penentuan nilai konsentrasi hambat minimum (khm) yaitu 1,25%, 2,5%, 5%, dan 10%. berdasarkan pengukuran zona bening pada hasil uji aktivitas antimikroba, seluruh isolat aktinobakteri memiliki daya hambat terhadap s. aureus dan c. albicans, namun tidak terhadap e. coli. isolat aktinobakteri aup-04 memiliki daya hambat tertinggi terhadap s. aureus dan c. albicans sehingga dilanjutkan untuk uji khm. nilai khm pada isolat aup-04 sebesar 1,25%. kata kunci: aktinobakteri, antimikroba, bakteri, cendawan, konsentrasi hambat minimum

Soil actinobacteria have the potential to produce antimicrobial compounds. Antimicrobial compounds are useful in the treatment of infectious diseases caused by bacteria and fungi. This study aimed to examine the potential of actinobacterial isolates from the soil of Ujong Pancu, Aceh Besar, as an antimicrobial producers. A total of 9 isolates of actinobacteria were evaluated as antimicrobial producer against the testing microbes Staphylococcus aureus, Escherichia coli, and Candida albicans by means of antagonist assay as well as minimum inhibitory concentration determination The results of the antimicrobial activity test based on clear zone measurement showed that all actinobacteria isolates had inhibition activity against S. aureus and C. albicans, but not against E. coli. AUP-04 actinobacteria isolate had the highest inhibition activity against S. aureus and C. albicans so it was continued for the MIC test. The MIC value in isolate AUP-04 was 1.25%. Keywords: Actinobacteria, antimicrobes, bacteria, fungi, minimum inhibitory concertration