Electronic Theses and Dissertation

Pasteurella multocida adalah bakteri gram negatif dan komersal serta paling banyak dibiakkan melalui air liur kucing. penularan bakteri pasteurella multocida bisa melalui gigitan maupun cakaran dari kucing. penelitian ini bertujuan untuk mengetahui tingkat infeksi bakteri pasteurella multocida pada kucing domestik yang mengalami infeksi sistem pernapasan. penelitian ini menggunakan metode cross sectional. sampel diambil dari 16 ekor kucing domestik yang mengalami infeksi sistem pernapasan. isolat swab gingiva kucing domestik dimasukkan kedalam tube yang berisi larutan pbs 1x. selanjutkan dilakukan tahap ekstraksi dna, amplifikasi dna dan visualisasi produk. hasil pengamatan dilakukan secara deskiptif. hasil pcr diperoleh 11 dari 16 ekor (69%) kucing domestik yang mengalami infeksi sistem pernapasan positif adanya pasteurella multocida. hasil penelitian ini dapat disimpulkan bahwa pasteurella multocida merupakan patogen oportunistik infeksi sistem pernapasan pada kucing domestik di klinik hewan legenda wisata cibubur. kata kunci: kucing domestik, infeksi sistem pernapasan, pasteurella multocida, pcr

Pasteurella multocida is Gram negative and commercial bacteria and is most commonly bred through cat saliva. Transmission of Pasteurella multocida bacteria can be through bites or scratches from cats. The study aimed to determine the level of Pasteurella multocida bacterial infection in domestic cats with respiratory system infections. The study used the cross sectional method. Samples were taken from 16 domestic cats that had respiratory system infections. A domestic cat gingival swab isolate is inserted into a tube containing a 1x PBS solution. Furthermore, the DNA extraction stage, DNA quantification and DNA amplification and product visualization stages are carried out. The results of observations are carried out deskly. PCR results were obtained by 11 out of 16 domestic cats who had a positive respiratory system infection with Pasteurella multocida. The results of this study can be concluded that Pasteurella multocida is an opportunistic pathogen of respiratory system infection in domestic cats at the Legenda Wisata Cibubur Veterinary Clinic. Keywords: Domestic cat, respiratory system infection, Pasteurella multocida, PCR