Electronic Theses and Dissertation

Helicobacter pylori adalah bakteri gram negatif yang memiliki bentuk spiral dan dapat ditemukan dalam saliva, plak gigi, serta feses. penelitian ini bertujuan untuk mengidentifikasi bakteri helicobacter pylori pada saliva kucing domestik yang mengalami gangguan pencernaan. pengambilan sampel dilakukan pada 16 ekor kucing domestik yang mengalami gangguan pencernaan di klinik hewan legenda wisata, cibubur dengan metode purposive sampling. swab saliva kucing dimasukkan ke dalam tube yang berisi larutan phospahate buffered saline 1x (pbs) dan disimpan di dalam freezer bersuhu -20°c. penelitian dengan menggunakan metode polymerase chain reaction (pcr) melewati proses ekstraksi dna dan kuantifikasi dna, amplifikasi dna menggunakan mesin konvensional dengan tiga tahapan yaitu denaturasi, annealing, dan extension, serta visualisasi produk amplifikasi. data hasil penelitian dianalisis secara deskriptif. hasil uji pcr menunjukkan 8 dari 16 sampel (50%) positif adanya helicobacter pylori. oleh sebab itu, pada saliva kucing domestik yang mengalami gangguan pencernaan dapat diidentifikasi adanya bakteri helicobacter pylori. kata kunci: helicobacter pylori, saliva, kucing domestik (felis domesticus), pcr

Helicobacter pylori is a Gram negative bacterium that has a spiral shape and can be found in saliva, dental plaque, and feces. This study aims to identify Helicobacter pylori bacteria in the saliva of domestic cats with indigestion. Sampling was carried out on 16 domestic cats with indigestion at the Legenda Wisata veterinary clinic, Cibubur used purposive sampling method. The swab saliva of cats was put into a tube containing 1x Phosphate Buffered Saline (PBS) solution and stored in a freezer at -20°C. Research using the polymerase chain reaction (PCR) method goes through the process of DNA extraction and DNA quantification, DNA amplification using a conventional machine with three stages, namely denaturation, annealing, and extension, as well as visualization of the amplification product. The results of the research data were analyzed descriptively. The outcome of the PCR test showed that 8 of the 16 samples (50%) were positive for Helicobacter pylori. Therefore, in the saliva of domestic cats with indigestion the presence of Helicobacter pylori bacteria can be identified. Keywords: Helicobacter pylori, saliva, domestic cat (Felis domesticus), PCR