Electronic Theses and Dissertation

ISOLASI DAN IDENTIFIKASI Salmonella sp. PADA REKTUM KELINCI (Oryctolagus cuniculus)

ABSTRAK
Kelinci (Oryctolagus cuniculus) menjadi salah satu agen reservoir yang dapat menyebarkan penyakit salmonellosis. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi Salmonella sp. pada rektum kelinci. Sampel diambil dari 15 ekor kelinci yang mengalami diare. Penelitian ini merupakan cross-sectional study yang dilakukan sesuai metode Carter. Spesimen swab rektum diinokulasi pada media Selenite Cystine Broth (SCB) dan diinkubasi dengan suhu 37°C selama 24 jam. Perubahan warna media menjadi oranye pada sampel dilanjutkan dengan penanaman pada media Salmonella Shigella Agar (SSA) untuk diamati koloni bakteri secara makroskopis. Pengamatan secara mikroskopis dilakukan dengan pewarnaan Gram. Selanjutnya, identifikasi spesies Salmonella sp. dengan uji biokimia, terdiri atas uji IMVIC (Indol, Methyl Red, Voges Proskauer, Sulfid Indol Motility, dan Simmon Citrate), Triple Sugar Iron Agar (TSIA), dan uji fermentasi gula (glukosa, mannitol, maltosa, laktosa, dan sukrosa). Diketahui bahwa, 46% (7/15 sampel) menunjukkan hasil positif Salmonella sp., 3/15 sampel (19,8%) Salmonella enterica serovar Thypimurium, 2/15 sampel (13,2%) Salmonella enteritidis, dan 2/15 sampel (13,2%) Salmonella arizonae. Oleh Sebab itu, dapat disimpulkan berdasarkan hasil identifikasi ditemukan Salmonella pada rektum kelinci diare yaitu S. enterica serovar Thypimurium, S. enteritidis, dan S. arizonae.
Kata kunci: Food borne disease, kelinci (Oryctolagus cuniculus), Non-Thypoid Salmonella (NTS), Salmonella sp. salmonellosis.

ISOLATION AND IDENTIFICATION OF Salmonella sp. IN RECTUM OF RABBIT (Oryctolagus cuniculus) ABSTRACT Rabbits (Oryctolagus cuniculus) are one of the reservoir agents that can spread salmonellosis. This study aims to isolate and identify Salmonella sp. in the rabbit rectum. Samples were taken from 15 rabbits with diarrhea. This research is a cross-sectional study conducted according to the Carter method. Rectal swab specimens were inoculated on Selenite Cystine Broth (SCB) media and incubated at 37°C for 24 hours. The change in the color of the media to orange in the sample was followed by planting on Salmonella Shigella Agar (SSA) media to observe bacterial colonies macroscopically. microscopically by Gram staining. Furthermore, identification the species of Salmonella sp. with a biochemical test, consisting of IMVIC test (Indol, Methyl Red, Voges Proskauer, Sulfid Indole Motility, and Simmon Citrate), Triple Sugar Iron Agar (TSIA), and sugar fermentation test (glucose, mannitol, maltose, lactose, and sucrose). Known that, 46% (7/15 samples) were positive for Salmonella sp., 3/15 samples (19.8%) were Salmonella enterica serovar Thypimurium, 2/15 samples (13.2%) Salmonella enteritidis, and 2/15 samples (13.2%) Salmonella arizonae. Therefore, it can be concluded that based on the identification results found Salmonella in the rectum of diarrhea rabbits, namely S. enterica serovar Thypimurium, S. enteritidis, and S. arizonae. Keyword: Food borne disease, rabbit (Oryctolagus cuniculus), Non-Thypoid Salmonella (NTS), Salmonella sp. salmonellosis.