Electronic Theses and Dissertation

Formulasi dan uji aktivitas losio tabir surya dari ekstrak dan fraksi daun kopi secara in vitro telah dilakukan. Penelitian ini bertujuan untuk mengetahui nilai SPF dari ekstrak, fraksi, dan sediaan losio dari daun kopi serta mengevaluasi sediaan yang dihasilkan. Pengujian nilai SPF menggunakan metode spektrofotometri UV-Vis. Nilai SPF Ekstrak etanol 70% pada konsentrasi 50, 100, dan 150 ppm secara berturut
yaitu 2,854; 5,321; dan 7,544; nilai SPF fraksi n-heksan pada konsentrasi 50, 100, dan 100 ppm secara berturut yaitu 1,519; 2,560; dan 3,294; nilai SPF fraksi etil asetat pada konsentrasi 50, 100, dan 150 ppm secara berturut yaitu 6,146; 15,035; dan 22,383; nilai SPF fraksi etanol-air pada konsentrasi 50, 100, dan 150 ppm secara berturut yaitu 3,048; 5,498; dan 7,801. Pembuatan sediaan losio menggunakan ekstrak atau fraksi dengan nilai SPF terbesar yaitu fraksi etil asetat konsentrasi 150 ppm. Nilai SPF F0 yaitu 0,765; nilai SPF losio F1 (0,03%) yaitu 9,865; dan nilai SPF
losio F2 (0,045%) yaitu 14,389. Secara organoleptik losio F0 berwarna putih susu, F1 berwarna krim muda, dan F2 berwarna krim dengan tekstur kental dan aroma khas basis yang sama untuk ketiga formula. Ketiga formula homogen dan memenuhi
kriteria daya sebar, daya lekat, dan pH. Hasil uji iritasi untuk ketiga formula tidak ada tanda-tanda terjadi iritasi. Hasil uji hedonik formula 1 memiliki warna yang agak disukai, tekstur yang agak disukai dan warna yang netral, sedangkan formula 2 memiliki warna yang netral, tekstur yang agak disukai dan aroma yang netral. Berdasarkan hasil penelitian sediaan losio tabir surya fraksi etil asetat layak untuk
diformulasikan karena memiliki nilai SPF kategori maksimum dan memenuhi semua evaluasi.
Kata kunci: daun kopi, Coffea canephora Pierre ex A.Froehner, fraksi etil asetat,
losio, tabir surya, nilai SPF

Formulations and in vitro sunscreen lotion activity testing from extract and fractions of coffee leaves has been done. This study aims to determine the SPF value of extracts, fractions, and lotion from coffee leaves and to do evaluation of the lotion. The method was used to determining SPF value was spectrophotometry UV-Vis. The SPF value of 70% ethanol extract at concentrations of 50, 100, and 150 ppm were 2,854; 5,321; and 7,544 respectively; the SPF value of n-hexane fraction at concentrations of 50, 100, and 100 ppm were 1.519; 2,560; and 3,294 respectively; the SPF value of ethyl acetate fraction at concentrations of 50, 100, and 150 ppm were 6,146; 15,035; and 22,383 respectively; the SPF value of ethanol-water fraction at concentrations of 50, 100, and 150 ppm were 3,048; 5,498; and 7,801 respectively. The lotion preparation was made using extract or fraction who had the greatest SPF value, namely ethyl acetate fraction at 150 ppm. The SPF value of F0 was 0,765; the SPF value of F1 (0,03%) was 9,865 and the SPF value of the F2 (0,045%) was 14,389. In organoleptic test, the colour of F0 was milky white, F1 was light cream, and F2 was cream with a thick texture and distinctive aroma from lotion base for all formulas. All formulas were homogeneous and have qualified for spreadability, stickiness, and pH tests. The irritation test results for all formulas did not show the signs of irritation. The results of the hedonic test of formula 1 have slightly preferred color, slightly preferred texture, and neutral color, while formula 2 has neutral color, slightly preferred texture, and neutral aroma. Based on the results of the study, the ethyl acetate fraction of sunscreen lotion is deserve to be formulated because it had a maximum category of SPF value and qualified all evaluations. Keywords: coffee leaf, Coffea canephora Pierre ex A.Froehner, ethyl acetate fraction, lotion, sunscreen, SPF value