Electronic Theses and Dissertation

Fusobacterium nucleatum merupakan salah satu bakteri yang terlibat dalam etiologi penyakit periodontal. F. nucleatum memiliki peran penting untuk menjembatani pembentukan koloni bakteri di rongga mulut yang memicu terjadinya periodontitis destruktif. Bakteri ini diketahui mengalami peningkatan jumlah pada kondisi hiperglikemia karena dianggap dapat menggunakan glukosa sebagai sumber nutrisi. Proses pemanfaatan glukosa pada bakteri terjadi dengan bantuan protein enzim seperti enzim glukokinase. Berdasarkan kondisi tersebut penelitian ini ingin melihat pemanfaatan glukosa oleh F. nucleatum ATCC 25586 dengan menguji pertumbuhannya dalam media yang diperkayai glukosa serta hubungannya dengan aktivitas protein secara FTIR. F. nucleatum ATCC 25586 dikultur dalam media yang diperkayai glukosa 2%, 1%, 0.75%, 0.5%, dan 0.25% lalu diukur pertumbuhannya dengan spektrofotometer dan aktivitas protein dengan FTIR pada 24 dan 48 jam masa inkubasi. Hasil penelitian menunjukkan F. nucleatum dapat memanfaatkan glukosa sebagai sumber nutrisi ditandai dengan terjadinya pertumbuhan dan adanya aktivitas protein. Pertumbuhan maksimal F. nucleatum terjadi pada konsentrasi 0.75% di waktu 24 jam. Tetapi hasil uji Kruskal-Wallis menunjukkan pertumbuhan dan aktivitas protein F. nucleatum tidak memiliki perbedaan signifikan berdasarkan kelima konsentrasi glukosa (p pertumbuhan =0.271 dan p protein =0.149). Pertumbuhan F. nucleatum tidak menunjukkan adanya korelasi dengan aktivitas protein berdasarkan uji korelasi Spearman (p=0.323). Aktivitas protein F. nucleatum tetap stabil pada berbagai tingkat pertumbuhan. Kesimpulannya yaitu glukosa dapat mempengaruhi pertumbuhan F. nucleatum meskipun pertumbuhan dan aktivitas protein bakteri tersebut tidak berbeda signifikan berdasarkan konsentrasi glukosa. F. nucleatum yang tumbuh pada berbagai konsentrasi glukosa memiliki aktivitas protein yang stabil.

Fusobacterium nucleatum is one of the bacteria involved in the etiology of periodontal disease. F. nucleatum has an important role in bridging the formation of bacterial colonies in the oral cavity that trigger destructive periodontitis. These bacteria are known to increase in number in hyperglycemic conditions because they are considered to be able to use glucose as a source of nutrition. The process of utilizing glucose in bacteria occurs with the help of protein enzymes such as glucokinase. Based on these conditions, this study wanted to examine the utilization of glucose by F. nucleatum ATCC 25586 by testing its growth in glucose-enriched media and its relationship with protein activity by FTIR. F. nucleatum ATCC 25586 was cultured in 2%, 1%, 0.75%, 0.5%, and 0.25% glucose-enriched media then its growth was measured by spectrophotometer and protein activity by FTIR at 24 and 48 hours of incubation. The results showed that F. nucleatum could utilize glucose as a source of nutrition, indicated by growth and protein activity. Maximum growth of F. nucleatum occurred at a concentration of 0.75% within 24 hours.However, the results of the Kruskal-Wallis test showed that the growth and protein activity of F. nucleatum had no significant difference based on the five glucose concentrations (p growth =0.271 and p protein =0.149). The growth of F. nucleatum did not show a correlation with protein activity based on the Spearman correlation test (p=0.323). Protein activity of F. nucleatum remains stable at various growth rates. The conclusion is that glucose can affect the growth of F. nucleatum although the growth and protein activity of these bacteria are not significantly different based on glucose concentration. F. nucleatum growing at various glucose concentrations had stable protein activity.