Electronic Theses and Dissertation

ABSTRAK

Latar Belakang: Penyakit Hirschsprung(PH) adalah keadaan kegagalan migrasi cephalocaudal sel saraf myenteric parasimpatis distal usus. Standar utama diagnosis PH adalah hasil pemeriksaan histopatologi dari biopsi rektal. Hasil biopsi dapat dilakukan melalui rectal suction biopsy/biopsi hisap rektum lalu dilakukan pemeriksaan secara histopatologi dengan pewarnaan hematoxilin eosin(H&E). Metode: Setelah hasil pemeriksaan pewarnaan H&E, maka dilanjutkan dengan pemeriksaan imunohistokimia acetylcolinesterase(AChE) dalam mengkonfirmasikan diagnosis PH. Penelitian ini menggunakan rancangan cross sectional dengan desain analitik observasional dan menilai uji diagnostik pemeriksaan AChE pada pasien dengan diagnosa klinis PH. Hasil: Pada penelitian didapatkan pada pemeriksaan AChE nilai sensitivitas 95%, nilai spesifisitas 100% dan nilai akurasi 96%. Dari penelitian ini juga didapatakn nilai Area Under Curve(AUC) 97,7%. Kesimpulan: Penelitian ini mendapatkan bahwa akurasi pemeriksaan AChE dalam menegakkan PH lebih baik dibandingkan dengan pewarnaan H&E.

Kata Kunci : Penyakit Hirschsprung, biopsi hisap rectum, imunoekspresi
acetylcolinesteras(AChE), pewarnaan hematoxilin eosin(H&E)

ABSTRACT Background: Hirschsprung's disease is a condition of failure of cephalocaudal migration of parasympathetic myenteric nerve cells in the distal intestine. The main standard for the Hirschsprung's disease diagnostic is the histopathological examination from the rectal biopsy. The results of the biopsy can be done through a rectal suction biopsy and then histopathological examination with hematoxylin eosin (H&E) staining is carried out. After the results of the H&E staining examination, then proceed with acetylcholinesterase (AChE) immunohistochemical examination to confirm Hirschsprung's disease. Method: This study used a cross sectional design with an observational analytic design and assessed the diagnostic test of AChE examination in patients with a clinical diagnosis of Hirschsprung's disease. Results: The results obtained on the AchE examination, the sensitivity value is 95%, the specificity value is 100% and the accuracy value is 96%. From this study, the Area Under Curve (AUC) value of 97.7% was also obtained. Conclusion: The conclusion of this study was that the accuracy of AChE examination in diagnostic of Hirschsprung's disease was better than H&E staining. Keywords: Hirschsprung Disease, Rectal suction biopsy, Acetylcolinesterase(AChE) immunoexpression, hematoxilin eosin(H&E) staining