Electronic Theses and Dissertation

ABSTRAK



Latar Belakang. Dislipidemia telah menjadi epidemi global dan merupakan masalah kesehatan utama di Indonesia sama seperti sindrom metabolik lainnya. Dislipidemia merupakan faktor risiko infertilitas terkait dengan adanya peningkatan radikal bebas, inflamasi, serta gangguan hormonal (steroidogenesis) yang berdampak pada penurunan kualitas sperma dan kerusakan blood testis barrier (BTB). Penurunan kualitas sperma ditemukan pada 30% kasus infertilitas. Sekitar 50-70 juta pasangan usia subur mengalami infertilitas, dan setiap tahun terdapat penambahan 2 juta kasus. Daun kelor (Moringa oleifera L) memiliki kandungan antiokisdan yang tinggi dan dapat digunakan dalam tatalaksana infertilitas. Tujuan penelitian ini adalah untuk mengetahui efek pemberian daun kelor terhadap kualitas sperma dan actin binding protein (ABP) testis pada tikus Rattus novergicus model dislipidemia.
Metode. Penelitian ini merupakan penelitian post test only control group design. Setelah dilakukan aklimatisasi selama 2 minggu, dilakukan pemberian pakan atherogenik dan perlakuan, dilakukan analisis sperma dan pemeriksaan ABP testis. Analisis data perbedaan antar kelompok dilakukan dengan uji Analisis of variance (ANNOVA) satu arah, kemudian dilanjutkan dengan uji post hoc Duncan. Analisis pengaruh perlakuan dilakukan dengan uji korelasi pearson dan uji regresi linear.
Hasil. Diet atherogenik dapat menginduksi dislipidemia pada semua kelompok perlakuan. Pemberian daun kelor 800 mg/kgBB/hari secara signifikan meningkatkan kualitas sperma dan ABP testis. Terdapat peningkatan jumlah sperma (126,60 ± 24,64 x 106/mm3), viabilitas (76,49 ± 9,05 %), dan penurunan morfologi abnormal (4,22 ± 2,22 %) pada kelompok perlakuan tersebut. Pemberian daun kelor dan titer ABP memiliki pengaruh yang sangat kuat terhadap jumlah sperma (R = 0,96), motilitas (R
= 0,93), viabilitas (R = 0,96), dan morfologi sperma (R = - 0,95).
Kesimpulan. Daun kelor memiliki potensi dalam tatalaksana infertilitas terkait dislipidemia. Ekstrak daun kelor dapat meningkatkan kualitas sperma dan ABP testis. Selain itu, ekstrak daun kelor dan ABP testis memiliki pengaruh yang sangat kuat terhadap kualitas sperma.

Kata kunci : Daun kelor, kualitas sperma, jumlah sperma, motilitas, viabilitas, morfologi, actin binding protein, Rattus novergicus

ABSTRACT Background. Dyslipidemia had become global epidemic and major health problem especially in Indonesia and related to metabolic syndrome. It had already known as risk factor of infertility due to many factors and related to increasing of reactive oxygen species, inflammation, hormonal (steroidogenesis) disturbance. As its result, it could decrease of sperm quality and damaged blood testis barrier especially testis actin binding protein. Decreasing of sperm quality was found in 30% infertility cases. About 50-70 milion couples had infertility problem, and every year there was 2 million infertility cases. Moringa oleifera L had high antioxidant content which could be used as its medication. The aim of this study was finding effect of Moringa oleifera L leaves extract on sperm quality and testis actin binding protein (ABP) on dyslipidemic male albino rats (Rattus novergicus). Method. A post test only control group design was performed on 4 groups to evaluate effect of Moringa oleifera L leaves extract. After acclimatization, atherogenic diet feeding was given to induced dyslipidemia. After 8 weeks, sperm analysis was performed and testis ABP was measured. Data were analyzed using one way analysis of variance (ANNOVA) test, post hoc Duncan test, pearson correlation test and linear regression test. Result. Atherogenic diet was able to induced dyslipidemia in all groups. Moringa oleifera L leaves with 800 mg/body weight daily dose had significant effect on sperm quality and testis actin binding proteins. It increased sperm count (126,60 ± 24,64 x 106/mm3), viability (76,49 ± 9,05 %), and decreased abnormal morphology (4,22 ± 2,22 %) of rats sperm. Moringa oleifera L leaves and ABP titer had very strong correlation with sperm quality. It increased sperm count (R = 0,96), motility (R = 0,93), viability (R = 0,96), and abnormal sperm morphology (R = - 0,952). Conclusion. Moringa oleifera L extract had potential effect for medication of infertility related dyslipidemia. It could increase sperm quality and testis actin binding proteins. It also had very strong effect along with testis ABP on sperm quality. Keyword : Moringa oleifera L, sperm quality, sperm count, motility, viability, morphology, testis, Actin binding protein, Rattus novergicus