Electronic Theses and Dissertation

Kulit nanas (Ananas comosus L. Merr) merupakan tanaman yang memiliki aktivitas antibakteri. Penelitian uji aktivitas sediaan gel hand sanitizer ekstrak kulit nanas telah dilakukan dengan tujuan mengetahui konsentrasi optimum ekstrak kulit nanas, parameter uji sediaan dan uji aktivitas sediaan terhadap bakteri S.aureus dan E.coli. Pengujian aktivitas antibakteri menggunakan metode difusi sumuran. Hasil karakterisasi simplisia menunjukkan kadar air 4,66%, kadar abu total 3,16%, kadar sari larut air 43,33% dan kadar sari larut etanol 40%. Ekstrak etanol diperoleh dengan metode maserasi dengan menghasilkan ekstrak berwarna coklat kekuningan. Hasil karakterisasi ekstrak menunjukkan kadar air 9,16%, kadar abu total 2,66%, kadar sari larut air 59,16% dan kadar sari larut etanol 61,66%. Hasil uji fitokimia ekstrak kulit nanas mengandung saponin, flavonoid dan tanin. Hasil uji aktivitas antibakteri ekstrak kulit nanas pada terhadap bakteri S.aureus dan bakteri E.coli konsentrasi 1,5% termasuk kategori sedang. Sedangkan pada konsentrasi 3% dan 4,5% menghasilkan zona hambat yang termasuk kategori kuat. Hasil uji evaluasi stabilitas sediaan gel hand sanitizer pada uji organoleptis dan homogenitas ketiga sediaan tidak mengalami perubahan. Hasil uji pH, viskositas, daya sebar dan daya lekat masih memenuhi syarat parameter sediaan yang baik. Hasil uji iritasi ketiga sediaan tidak menimbulkan iritasi. Hasil uji aktivitas antibakteri sediaan gel hand sanitizer pada F1 terhadap bakteri S.aureus and E.coli termasuk kategori kuat yaitu 21,24 mm dan kategori sedang yaitu 20,88 mm. sedangkan zona hambat yang dihasilkan pada F2 termasuk kategori kuat yaitu 24,13 mm and 24,06 mm. Berdasarkan penelitian tersebut dapat disimpulkan semua sediaan memenuhi syarat parameter sediaan yang baik serta F1 dan F2 memiliki aktivitas antibakteri terhadap bakteri S.aureus dan E.coli.
Kata kunci : Antibakteri, Gel Hand Sanitizer, Kulit Nanas.

Pineapple peel (Ananas comosus L. Merr) is a plant that has antibacterial activity. Research on the activity test of the hand sanitizer gel preparation of peel extract has been carried out with the aim of knowing the optimum concentration of pineapple peel extract, preparation test parameters and activity testing against S. aureus and E. coli bacteria. Antibacterial activity test using well diffusion method. The results of simplicia characterization showed that the water content was 4.66%, the total ash content was 3.16%, the water soluble extract content was 43.33% and the ethanol soluble essence was 40%. Ethanol extract was obtained by maceration method to produce a brown extract. The results of the characterization of the extract showed that the water content was 9.16%, the total ash content was 2.66%, the water soluble extract content was 59.16% and the ethanol soluble extract was 61.66%. The results of the phytochemical test of pineapple peel extract contain saponins, flavonoids and tannins. The results of the antibacterial activity of pineapple peel extract on S.aureus and E.coli bacteria at a concentration of 1.5% were in the medium category. While at concentrations of 3% and 4.5% resulted in a zone of inhibition which is included in the strong category. The results of the evaluation of the hand sanitizer gel preparation in the organoleptic test and the homogeneity of the three preparations did not change. The test results of pH, viscosity, spreadability and adhesion still meet the requirements of good preparation parameters. The test results of the three preparations did not cause irritation. The results of the antibacterial activity test for hand sanitizer gel preparations in F1 against S.aureus and E.coli bacteria were in the strong category namely 21,24 mm and the medium category namely 20,88 mm. While the inhibition zones produced in F2 are in the strong category namely 24,13 mm and 24,06 mm. Based on these studies, it can be ensured that all preparations meet the requirements of good preparation parameters and F1 and F2 have antibacterial activity against S.aureus and E.coli bacteria. Keywords: Antibacterial, Gel Hand Sanitizer, Pineapple peel.