Electronic Theses and Dissertation

ABSTRAK
Pengukuran hormon testosteron secara non invasif dalam sampel feses membutuhkan kit ELISA spesifik. Penelitian ini bertujuan untuk menguji akurasi, presisi dan sensitivitas kit ELISA epiandrosteron untuk mengukur metabolit testosteron pada feses rusa sambar. Penelitian ini menggunakan 26 sampel feses yang dikoleksi dari tiga rusa sambar di Taman Rusa Lamtanjong, Aceh Besar. Sampel feses kemudian diekstraksi dan konsentrasi metabolit testosteron diukur menggunakan metode ELISA. Uji akurasi dilakukan dengan cara 1) menghitung % recovery rate dari sampel dan standar epiandrosteron, 2) uji paralellism , dan 3) mengukur konsentrasi metabolit testosteron pada rusa jantan dan betina. Uji presisi dilakukan dengan menghitung %coefficient variation/CV intra-assay dan inter-assay dari low quality control (QC low) dan high quality control (QC high). Sensitivitas dihitung dari konsentrasi terkecil standar 0. Data dianalisis dengan uji kruskal wallis dan Mann Whitney U Test. Hasil penelitian diperoleh nilai % recovery rate sebesar 102,44±12,51%. Hasil uji parallelism didapatkan kurva sampel parallel dengan kurva standar epiandrosteron. Konsentrasi metabolit testosteron pada rusa jantan dewasa lebih tinggi secara signifikan dibandingkan dengan konsentrasi metabolit testosteron pada rusa betina dewasa (P

ABSTRACT Non-invasive measurement of the hormone testosterone in fecal samples requiries specific ELISA kit. This study aims to test the accuracy, precision and sensitivity of the epiandrosteron ELISA kit which is used for the measurement of testosterone metabolites in sambar deer feces. This study was used 26 fecal samples collected from three of sambar deer living in Lamtanjong Deer Park, Aceh Besar. Fecal samples were extracted and the testosterone metabolite concentration was measured using the ELISA method. The accuracy test was carried out by 1) calculating the % recovery rate from fecal and standard epiandrosterone, 2) parallelism test, and 3) measuring the concentration of testosterone metabolites in male and female deer. The precision test was carried out by calculating the % coefficient variation/CV of intra-assay and inter-assay of low quality control (low QC) and high quality control (high QC). Sensitivity was calculated from the smallest standard concentration of 0. Data were analyzed by Kruskal Wall and Mann Whitney U Tests. The results showed that the % recovery was 102.44±12.51%. The results of the parallelism test showed that the sample curve was parallel to the epiandrosterone standard curve. The concentration of testosterone metabolites in adult male deer was significantly higher than the concentration of testosterone metabolites in adult female deer (P