Electronic Theses and Dissertation

ABSTRAK
Hormon estrogen pada hewan jantan berperan dalam spermatogenesis pada testis dan maturasi spermatozoa pada duktus epididimis setelah berikatan dengan reseptor estrogen. Penelitian ini bertujuan mendeteksi sebaran reseptor estrogen alpha (ER?) pada jaringan duktus epididimis sapi aceh menggunakan teknik imunohistokimia (IHK). Sampel yang digunakan adalah lima pasang organ epididimis (dexter et sinister) yang dikoleksi dari lima ekor sapi aceh jantan dewasa berumur 2-3 tahun. Sampel diproses menjadi preparat histologi dan diwarnai dengan pewarnaan IHK metode avidin biotin complex peroxidase (ABC method). Ekspresi ER? dianalisis menggunakan metode intensity score sedangkan untuk mengetahui perbedaan ekspresi ER? pada jaringan duktus epididimis sapi aceh dilakukan uji Kruskal-Wallis dan dilanjutkan dengan uji Mann Whitney U Test. Hasil penelitian menunjukkan lokasi sebaran ER? dapat dideteksi pada kaput, korpus, dan kauda epididimis yang meliputi sel-sel epitel, membran basal, dan serabut otot polos. Berdasarkan uji statistik terdapat perbedaan nyata (P0,05) antara ketiga bagian epididimis sapi aceh. Dapat disimpulkan bahwa sebaran ER? ditemukan pada sel epitel, membran basal, dan serabut otot polos pada kaput, korpus, dan kauda epididimis sapi aceh dengan ekspresi imunoreaktivitas yang berbeda.

Kata kunci: duktus epididimis, imunohistokimia, reseptor estrogen alpha, sapi aceh

ABSTRACT
The hormone estrogen in male animals plays a role in spermatogenesis in the testes and maturation of spermatozoa in the ductus epididymis after binding to the estrogen receptor. This study aims to detect the distribution of estrogen receptor alpha (ER?) in the ductus epididymis of Aceh cattle using immunohistochemical (IHK) techniques. The samples used were five pairs of epididymal organs (dexter et sinister) which were collected from five adult male Aceh cattle aged 2-3 years. All samples were processed into histological preparations and stained with IHK staining using avidin biotin complex peroxidase (ABC method) method. ER? expression was analyzed using the intensity score method, while to determine differences in ER? expression in the ductus epididymal tissue of Aceh cattle, the Kruskal-Wallis test was performed and if there were differences, the Mann Whitney U test was used. The results showed that the location of ER? distribution could be detected in the caput, corpus, and cauda epididymis which included epithelial cells, basement membrane, and smooth muscle fibers. Based on statistical tests, there was a significant difference (P0.05) in ER? expression between the three parts of the epididymis of Aceh cattle. It can be concluded that the distribution of ER? was found in epithelial cells, basement menbranes, and smooth muscle fibers in the caput, corpus, and cauda epididymis of aceh cattle with different expressions of immunoreactivity.

Keywords: aceh cattle, ductus epididymis, estrogen receptor alpha, immunohistochemistry

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