Electronic Theses and Dissertation

DETEKSI CEMARAN Escherichia coli PADA UD ANG VANAME (Litopenaeus vannamei) DI TAMBAK KECAMATAN
LHOONG ACEH BESAR


ABSTRAK

Udang vaname merupakan salah satu produksi andalan perikanan indonesia yang memiliki nilai jual yang tinggi dan juga jenis udang yang banyak ditemukan dan dimanfaatkan di Aceh. Udang vaname pada tambak Kecamatan Lhoong Aceh Besar mengalami penurunan produktvitas sehingga perlu dilakukan penanganan yang baik agar produktivitas udang meningkat. Penelitian ini bertujuan untuk mendeteksi adanya cemaran Escherichia coli pada udang vaname (Litopenaeus vannamei) di tambak Kecamatan Lhoong Aceh Besar. Sampel penelitian berjumlah 10 ekor udang vaname. Deteksi E. coli menggunakan eosin methylen blue agar (EMBA) dan data hasil pengamatan dianalisis secara deskriptif. Hasil penelitian ini menunjukkan bahwa pada 10 sampel udang menunjukkan hasil negatif E. coli namun positif Coliform, karena pada media EMBA tidak terlihat adanya warna hijau metalik melainkan warna merah muda mengindikasikan keberadaan Coliform, dengan jumlah koloni terendah 4,2x10² cfu/g dan jumlah koloni tertinggi 1,3x104 cfu/g. Kesimpulan dari penelitian ini adalah tidak ditemukan adanya cemaran E. coli namun terdapat keberadaan Coliform indikator dugaan adanya bakteri patogen lain yang mencemari udang vaname,
Keywords: Litopenaeus vannamei, Escherichia coli, Coliform, udang vaname



DETECTION OF Escherichia coli CONTAMINATION IN SHRIMP VANAME (Litopenaeus vannamei) IN THE POND OF LHOONG DISTRICT
ACEH BESAR


ABSTRACT

Vannamei shrimp is one of the mainstay products of Indonesian fisheries which has a high selling value and is also a type of shrimp that is widely found and used in Aceh. The vaname shrimp in the pond of Lhoong District Aceh Besar pond has decreased in productivity so that it needs to be solved properly so that shrimp productivity increases. This study aims to detect Escherichia coli contamination on vaname shrimp (Litopenaeus vannamei) in the pond of Lhoong Aceh Besar pond. The research sample consisted of 10 vaname shrimp. E. coli detection using eosin methylen blue agar (EMBA) and data resulting from observations were analyzed descriptively. The results of this study indicate that 10 shrimp samples showed negative E. coli results but positive Coliform, because in the EMBA media there was no metallic green color but pink color, with the lowest number of colonies 4.2x10² cfu / g and the highest number of colonies 1, 3x104 cfu / g. This study concludes of this study is that no E. coli contamination was found, but there was the presence of Coliform bacteria and it had exceeded the maximum limit of Colifrom contamination in vaname shrimp. Thus the presence of Coliform bacteria is an indicator of the suspected presence of other pathogenic bacteria that contaminate vaname shrimp,
Keywords: Litopenaeus vannamei, Escherichia coli, Coliform, vaname shrimp

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