Electronic Theses and Dissertation

ABSTRAK
Unggas memiliki kulit yang berfungsi melindungi permukaan tubuh secara mekanik terdapat kemungkinan masuknya zat-zat asing, mengatur temperatur tubuh, dan sebagai kelenjar ekskresi. Kulit unggas memiliki struktur yang lebih tipis dibandingkan dengan kulit spesies lain seperti mamalia. Struktur kulit unggas secara histologis pada umumnya dapat dibagi atas 3 (tiga) yaitu lapisan epidermis, dermis (corium), dan hipodermis (subcutis). Penelitian ini bertujuan mengetahui kepadatan serabut kolagen pada kulit ayam kampung, bebek, dan burung merpati masing-masing sebanyak tiga ekor dengan jenis kelamin jantan. Kulit regio ventral abdomen dari masing-masing sampel kemudian diproses hingga menjadi sediaan histologi dengan ketebalan 5 μm, selanjutnya diwarnai menggunakan pewarnaan picro sirius red (PSR). Kepadatan serabut kolagen disajikan dalam bentuk skoring dan data skoring dianalisis menggunakan uji Kruskall Wallis dan apabila terdapat perbedaan dilanjutkan dengan uji Mann-Whitney. Hasil uji Kruskall Wallis menunjukkan P = 0,00 (P

ABSTRACT Poultry have skin that functions to mechanically protect the body surface from the possible entry of foreign substances, regulate body temperature, and as an excretory gland. Poultry skin has a thinner structure compared to the skin of other species such as mammals. Histologically, poultry skin structure can generally be divided into 3 (three) layers: epidermis, dermis (corium), and hypodermis (subcutis). This study aims to determine the density of collagen fibers in the skin of domestic fowl, ducks, and doves, each with three males. The skin of the ventral abdominal region of each sample was then processed into histology preparations with a thickness of 5 μm, then stained using picro sirius red (PSR) staining. The density of collagen fibers was presented in the form of scoring and scoring data were analyzed using the Kruskall Wallis test and if there was a difference, it was continued with the Mann-Whitney test. Kruskall Wallis test results showed P = 0.00 (P < 0.05) which means there is a very real difference between birds, while the results of the Mann-Whitney test showed that all types of poultry have a very real difference in the density of collagen fibers between birds with a significant value (P < 0.05). Based on the research conducted, it was concluded that there was a difference in collagen fiber density in the three birds. The highest density of collagen fibers is in free-range domestic fowl, doves, and ducks. Keywords: Collagen connective tissue, picro sirius red staining, skin and poultry.