Electronic Theses and Dissertation

Heat Shock Protein 70 (HSP70) merupakan molekul pendamping (chaperone) yang memiliki peran
sebagai proteksi sel dan penanda biologis (biomarker) yang terbentuk dalam kondisi stres atau trauma
intrinsik maupun ekstrinsik. Infeksi virus merupakan salah satu kondisi yang dapat menyebabkan
kerusakan sel, salah satunya adalah infeksi virus Newcastle Disease (ND) yang terjadi pada unggas.
Unggas seperti ayam dan itik dapat mengalami gangguan berupa kerusakan pada sistem saraf selama
masa infeksi virus ND. Penelitian ini bertujuan untuk mengetahui ada tidaknya dan perbedaan derajat
ekspresi HSP70 pada otak ayam kampung dan itik yang diinfeksikan isolat virus ND virulen pada
setiap hari pengamatan. Sampel penelitian berupa otak ayam kampung dan itik dari 4 kelompok
percobaan yaitu, AC-A (kelompok ayam kampung), AC-I (kelompok itik) yang diinfeksikan virus
ND isolat virus Ducks/Aceh Besar IND/2013/eoAc080721), K-A (kelompok kontrol negatif ayam
kampung) dan K-I (kelompok kontrol negatif itik) yang diinokulasikan PBS. Semua kelompok
diinokulasikan secara intraorbital. Sampel otak dikoleksi pada hari ke 1,3,5 dan 7 pasca infeksi (pi).
Derajat ekspresi HSP70 dideteksi dengan metode pewarnaan imunohistokimia menggunakan antibodi
primer Anti-HSP70. Data hasil ekspresi HSP70 dianalisis secara deskriptif dan diskoring berdasarkan
derajat sel imunopositif dengan kategori normal, sedikit, sedang dan banyak. Hasil penelitian
menunjukkan HSP70 terekspresi pada sitoplasma dan inti sel neuron dan sel glia. Derajat sel
imunopositif pada otak ayam kampung menunjukkan kategori sedang pada hari ke 1, 5 dan 7 pi.
Derajat sel imunopositif pada otak itik menunjukkan kategori sedikit pada setiap hari pengamatan.
Derajat sel imunopositif pada otak ayam kampung dan itik kontrol negatif menunjukkan kategori
normal pada setiap hari pengamatan. Berdasarkan hasil penelitian yang telah dilakukan, maka dapat
disimpulkan bahwa terdapat ekspresi HSP70, dengan derajat ekspresi lebih banyak pada otak ayam
kampung daripada itik.
Kata kunci : ayam kampung, HSP70, itik, Newcastle Disease, otak

Heat Shock Protein 70 (HSP70) is a chaperone molecule which has a role as cell protection and a biological marker (biomarker) which is formed in conditions of intrinsic or extrinsic stress or trauma. Viral infection is a condition that can cause cell damage, one of which is Newcastle Disease (ND) virus infection which occurs in poultry. Poultry such as chickens and ducks can experience problems in the form of damage to the nervous system during ND virus infection. This study aims to determine the presence or absence and differences in the degree of HSP70 expression in the brains of free-range chickens and ducks infected with virulent ND virus isolates on each day of observation. The research samples consisted of brains from free-range chickens and ducks from 4 experimental groups, namely, AC-A (free-range chicken group), AC-I (duck group) infected with the ND virus isolate Ducks/Aceh Besar IND/2013/eoAc080721), K-A (negative control of free-range chickens group) and K-I (negative control of ducks group) which were inoculated with PBS. All groups were inoculated intraorbitally. Brain samples were collected on days 1,3,5 and 7 post infection (pi). The degree of HSP70 expression was detected by immunohistochemical staining method using Anti-HSP70 primary antibody. Data on HSP70 expression results were analyzed descriptively and scored based on the degree of immunopositive cells in the categories normal, few, moderate and many. The results showed that HSP70 was expressed in the cytoplasm and nucleus of neuronal cells and glial cells. The degree of immunopositive cells in the brains of free-range chickens showed a moderate category on days 1, 5 and 7 pi. The degree of immunopositive cells in the duck's brain showed a slight category on each day of observation. The degree of immunopositive cells in the brains of free-range chickens and negative control ducks showed the normal category on each day of observation. Based on the results of research that has been carried out, it can be concluded that there is expression of HSP70, with a greater degree of expression in the brains of free-range chickens than ducks. Key words:, free-range chickens, HSP70, ducks, Newcastle Disease, brain