Electronic Theses and Dissertation

Telah dilakukan sintesis nanopartikel tembaga dengan metode green synthesis menggunakan ekstrak air daun sirsak (Annona muricata L) sebagai agen pereduksi dan stabilisator. Hasil penentuan total phenolic content (TPC) dengan metode Folin-Ciocalteu dan total flavonoid content (TFC) menggunakan metode kolorimetri AlCl3 masing-masing sebesar 75,52 GAE/g dan 0,173 QE/g. Kandungan fenolik dan flavonoid yang terdapat pada ekstrak air daun sirsak bertanggung jawab untuk pembentukan nanopartikel tembaga. Hasil reaksi reduksi ekstrak air daun sirsak terhadap ion Cu2+ dikonfirmasi dengan menggunakan Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) dan Transmisssion Electron Microscopy (TEM). Hasil karakterisasi menunjukkan bahwa nanopartikel yang terbentuk adalah Cu, CuO, Cu2O dan CuO2. Analisis FTIR memperlihatkan gugus OH berperan dalam proses reduksi logam. Hasil sintesis nanopartikel tembaga menunjukkan aktivitas antimikroba terhadap Escherichia coli, Staphylococcus aureus dan Candida albicans dengan daya hambat tertinggi pada konsentrasi 20%.

The synthesis of copper nanoparticles has been carried out using the green synthesis method with soursop leaf extract (Annona muricata L) as a reducing and stabilizing agent. The results of the total phenolic content (TPC) determination using the Folin-Ciocalteu method and the total flavonoid content (TFC) using the AlCl3 colorimetric method were 75.52 GAE/g and 0.173 QE/g, respectively. The phenolic and flavonoid content in the soursop leaf extract is responsible for the formation of copper nanoparticles. The reduction reaction of soursop leaf extract against Cu2+ ions was confirmed using Fourier Transform Infrared (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM). Characterization results showed that the formed nanoparticles are Cu, CuO, Cu2O, and CuO2. FTIR analysis showed that the OH group plays a role in the metal reduction process. The synthesized copper nanoparticles exhibited antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans with an highest inhibition zone at a 20% concentration.