Electronic Theses and Dissertation

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian minyak nilam aceh (Pogostemon cablin Benth.) fraksi ringan (MNAFR) secara oral terhadap makroskopis dan mikroskopis hati tikus (Rattus norvegicus Berkenhout) setelah diinduksi depresi. Metode yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) yang terdiri dari 5 perlakuan dan 5 ulangan. Induksi depresi dilakukan menggunakan metode uji Tail Suspension Test (TST), pengujian dilakukan sekali sehari dengan durasi 6 menit selama 14 hari. Perlakuan terdiri dari kontrol (K0) pemberian CMC-Na (K-), sertraline (K+), MNAFR 20 mg/200 g BB (P1) dan MNAFR 40 mg/200 g BB (P2) dilakukan pada hari ke 15 sampai hari ke-28. Pembuatan sediaan histologi hati menggunakan metode parafin dan pewarnaan Hematoksilin dan Eosin (HE). Parameter yang diukur yaitu perilaku hewan coba setelah diinduksi depresi, pengamatan makroskopis hati berupa perubahan warna dan berat hati serta pengamatan mikroskopis berupa nekrosis dan degenerasi hepatosit. Data rerata perilaku setelah induksi depresi diuji secara deskriptif. Data warna hati dianalisis dengan Kruskall Wallis dan uji lanjut ManWhitney, sedangkan data berat hati analisis dilakukan dengan ANOVA dan uji lanjut dengan Least Significant Difference (LSD). Nekrosis dan degenerasi dianalisis menggunakan ANOVA dengan taraf signifikan 5% dan diuji lanjut dengan uji berganda Duncan. Hasil penelitian menunjukkan pemberian minyak nilam aceh fraksi ringan dosis 20 mg/200 g BB memberikan pengaruh secara mikroskopis berupa nekrosis hepatosit pada hati tikus, sedangkan pada dosis 40 mg/200 g BB minyak nilam aceh fraksi ringan sudah memberikan pengaruh yang setara dengan sertraline terhadap pengaruh nekrosis.

This research aims to determine the effect of oral administration of Aceh patchouli (Pogostemon cablin Benth.) light fraction (APOLF) oil on the liver macroscopic and microscopic of rats (Rattus norvegicus Berkenhout) after being induced by depression. The method used in this research was a Completely Randomized Design (CRD) which consisted of 5 treatments and 5 replications. Depression induction was carried out using the Tail Suspension Test (TST) test method, the test was carried out once a day with a duration of 6 minutes for 14 days. Treatment consisted of control (K0) administration of CMC-Na (K-), sertraline (K+), APOLF 20 mg/200 g BW (P1) and APOLF 40 mg/200 g BW (P2) carried out on day 15 to day -28. Liver histology preparations were made using the paraffin method and Hematoxylin and Eosin (HE) staining. The parameters measured were the behavior of experimental animals after being induced by depression, macroscopic observations of the liver in the form of changes in liver color and liver weight and microscopic observations in the form of necrosis and degeneration of hepatocytes. Average behavioral data after depression induction were tested descriptively. Liver color parameters were analyzed using Kruskall Wallis and the Man Whitney advanced test, while for liver weight the analysis was carried out using ANOVA and further tests with Least Significant Difference (LSD). Necrosis and degeneration were analyzed using ANOVA with a significance rate of 5% and further tested using Duncan's multiple test. The results of the study showed that administration of light fraction Acehnese patchouli oil at a dose of 20 mg/200 g BW had a microscopic effect in the form of hepatocyte necrosis in rat livers, where as at a dose of 40 mg/200 g BW light fraction Aceh patchouli oil had an effect equivalent to sertraline on effect of necrosis.