Electronic Theses and Dissertation

ABSTRAK
Bakteri pada ekosistem mangrove memiliki peran penting dalam proses penguraian. Tujuan penelitian ini adalah untuk menganalisis jumlah populasi bakteri selulolitik dan karakteristik bakteri pada tanah dengan uji makroskopis dan mikroskopis serta uji aktivitas enzim selulase pada ekosistem mangrove. Metode pengambilan sampel pada penelitian ini dilakukan dengan purposive sampling. Sampel yang diambil dengan tiga kali pengulangan pada tiga lokasi. Hasil penelitian menunjukkan bahwa jumlah populasi bakteri tertinggi yaitu 1,10 x 102 dan yang paling terendah adalah 0,40 x 103. Pengamatan makroskopis meliputi 6 isolat yang telah dimurnikan, rata-rata bentuk isolat yang ditemukan adalah bundar. Karakteristik elevasi koloni banyak dijumpai yaitu cembung, karakteristik tepi koloni banyak dijumpai yaitu berbentuk rata dan berdasarkan karakteristik dilihat dari pigmen berwarna krem, krem keputihan dan kuning. Pengamatan mikroskopis meliputi bentuk sel, warna sel dan pewarnaan gram. Berdasarkan pengamatan mikroskopis memperlihatkan bahwa bentuk yang dijumpai adalah basil. Karakteristik pewarnaan gram yang dijumpai yaitu gram positif. Berdasarkan hasil penelitian morfologi (makroskopis dan mikroskopis) menunjukkan bahwa isolat bakteri tersebut diduga termasuk dalam genus Bacillus sp. Hasil aktivitas enzim menunjukkan bahwa dari 6 isolat yang peroleh, hanya 3 isolat yang memiliki kemampuan menghasilkan enzim selulase. Isolat BMB 1.2 memiliki indeks selulolitik tertinggi yaitu 1,81 mm. Isolat BMI 1.4.2 memiliki nilai indeks selulolitik terendah yaitu 0,52 mm.
Kata Kunci : Bakteri, isolat, populasi, tanah, ekosistem mangrove.

ABSTRACT Bacteria in the mangrove ecosystem have an important role in the decomposition process. The aim of this research is to analyze the population of cellulolytic bacteria and the characteristics of bacteria in the soil using macroscopic and microscopic tests as well as cellulase enzyme activity tests in the mangrove ecosystem. The sampling method in this research was purposive sampling. Samples were taken three times in three locations. The results showed that the highest bacterial population was 1.10 x 102 and the lowest was 0.40 x 103. Macroscopic observations included 6 isolates that had been purified, the average shape of the isolates found was round. The elevation characteristics of colonies are often found, namely convex, the characteristics of the edges of colonies are often found, namely they are flat and based on the characteristics seen from the pigment colored cream, whitish cream and yellow. Microscopic observations include cell shape, cell color and gram staining. Based on microscopic observations, it shows that the form found is a Bacillus. The characteristics of the gram stain found are gram positive. Based on the results of morphological research (macroscopic and microscopic), it show that the bacterial isolate is thought to belong to the genus Bacillus sp. The enzyme activity results showed that of the 6 isolates obtained, only 3 isolates had the ability to produce cellulase enzymes. BMB 1.2 isolate had the highest cellulolytic index, namely 1.81 mm. Isolate BMI 1.4.2 had the lowest cellulolytic index value, namely 0.52 mm. Keywords : Bacteria, isolates, populations, soil, mangrove ecosystem.