Electronic Theses and Dissertation

Monosodium Glutamat (MSG) merupakan zat kimia yang mengandung glutamat dalam bentuk bebas yang tidak terikat pada molekul protein, sehingga mengonsumsi MSG secara berlebihan dapat membentuk radikal bebas yang dapat bermanifestasi terhadap kerusakan ginjal. Black garlic merupakan produk olahan bawang putih yang difermentasi pada suhu 70°C selama 17 hari sehingga memiliki kandungan antioksidan 25 kali lebih tinggi dibandingkan bawang putih biasa yang berpotensi mencegah terjadinya stress okidatif. Penelitian ini bertujuan untuk mengetahui efek ekstrak etanol black garlic terhadap ginjal tikus putih yang diinduksi MSG. Penelitian ini adalah merupakan penelitian eksperimental menggunakan rancangan acak lengkap (RAL) dengan sampel ginjal tikus yang berasal dari 5 kelompok perlakuan dan 3 ulangan yang terdiri atas kontrol negatif (P0), kontrol positif (P1) yang diinduuksi MSG dan kelompok terapi P2, P3 dan P4 yang diinduksi MSG 8 mg/kg BB dan dilanjutkan dengan pemberian ekstrak etanol black garlic dengan dosis 200 mg/kg BB, 400 mg/kg BB dan 600 mg/kg BB selama 28 hari. Setelah itu organ ginjal dikoleksi dan dibuat preparat histopatologi. Hasil kelompok P0 ditemukan gambaran glomerulus dan tubulus yang normal, kelompok P1 ditemukan pembengkakan, glomerulus mengalami adhesi, hiperemi dan pengecilan lumen tubulus, pada kelompok P2 ditemukan glomerulus dan tubulus yang normal dan hiperemi, pada kelompok P3 ditemukan glomerulus adhesi, penumpukan sel radang dan hiperemi, pada kelompok P4 ditemukan glomerulus adhesi dan lumen tubulus menyempit. Kesimpulan menunjukkan bahwa pemberian ekstrak etanol black garlic dengan dosis 200 mg/kg bb dapat meregenerasi ginjal yang rusak akibat induksi MSG.

Monosodium Glutamate (MSG) is a chemical that contains glutamate in free form which is not bound to protein molecules, so consuming excessive amounts of MSG can form free radicals which can manifest in kidney damage. Black garlic is a processed garlic product that is fermented at 70°C for 17 days so it has an antioxidant content 25 times higher than regular garlic which has the potential to prevent oxidative stress. This study aims to determine the effect of black garlic ethanol extract on the kidneys of white rats induced by MSG. This research was an experimental study using a completely randomized design (CRD) with rat kidney samples from 5 treatment groups and 3 replications consisting of negative control (P0), positive control (P1) induced by MSG and therapy groups P2, P3 and P4 was induced by MSG at 8 mg/kg body weight and continued with administration of black garlic ethanol extract at a dose of 200 mg/kg, 400 mg/kg body weight and 600 mg/kg body weight for 28 days. After that, the kidney organs were collected and histopathology preparations were made. The results of group P0 found a normal picture of the glomerulus and tubules, group P1 found swelling, the glomerulus had adhesions, hyperemia and reduction of the tubule lumen, in group P2 the glomerulus and tubules were found to be normal and hyperemic, in group P3 the glomerulus found adhesions, accumulation of inflammatory cells and hyperemia, in the P4 group, glomerular adhesions were found and the tubular lumen was narrowed. The conclusion shows that administration of black garlic ethanol extract at a dose of 200 mg/kg bw can regenerate kidneys damaged due to MSG induction.