Electronic Theses and Dissertation

Kontaminasi tangan merupakan salah satu sumber dari pencemaran bakteri E. coli. Penelitian ini bertujuan untuk menguji resistensi Escherichia coli penghasil ESBL terhadap antibiotik pada tangan pedagang daging ayam Broiler di Pasar Al-Mahirah Kota Banda Aceh. Metode deteksi E. coli mengacu pada Observasi Cross Sectional dan pengujian resistensi bakteri E. coli terhadap antibiotik dilakukan dengan metode disc diffusion (difusi cakram). Penelitian ini menggunakan 47 sampel telapak tangan pedagang daging ayam broiler. Antibiotik yang digunakan yaitu ampisilin, ceftazidime, cefotaxim, kanamisin, trimethoprim-sulfametoksazol dan eritromisin. Data dianalisis secara deskriptif. Hasil penelitian ini menunjukkan bahwa dari 47 sampel tangan 6 diantaranya positif tercemar E. coli penghasil ESBL. Tingkat resistensi E. coli terhadap antibiotik adalah ampisilin 6/6 (100%), eritromisin 5/6 (83%), cefotaxim 6/6 (100%), seftazidime 6/6 (100%), kanamisin 2/6 (33%), dan trimethoprim-sulfametoksasol 2/6 (33%), hasil ini menunjukkan tingkat resistensi yang bervariasi terhadap antibiotik. Berdasarkan hasil penelitian dapat disimpulkan bahwa bakteri E. coli penghasil ESBL ditemukan pada tangan pedagang daging ayam broiler di Pasar Al-Mahirah Kota Banda Aceh dan E. coli penghasil ESBL resisten terhadap antibiotik ampisilin, cefotaxim, seftazidime, kanamisin, eritromisin, dan trimethoprim-sulfametoksazol.
Kata kunci: Antibiotik, ESBL, Resistensi, Tangan

Hand contamination is one source of Escherichia coli bacterial contamination. This study aims to test the resistance of Escherichia coli producing Extended Spectrum Beta-Lactamase to antibiotics on the hands of Broiler chicken meat traders at Al-Mahirah market, Banda Aceh city. The detection method of Escherichia coli refers to Cross Sectional Observation and testing the sensitivity of Escherichia coli bacteria to antibiotics is carried out by the disc diffusion method. This study used 47 samples of chicken meat traders' hands. Antibiotics used were ampicillin, ceftazidime, cefotaxim, kanamycin, trimethoprim-sulfamethoxazole and erythromycin. Data were analyzed descriptively. The results of this study indicated that out of 47 hand samples, 6 were positive for ESBL-producing Escherichia coli. Antibiotic resistant level of ampicillin 6/6 (100%), erythromycin 5/6 (83%), cefotaxim 6/6 (100%), cefazidime 6/6 (100%), kanamycin 2/6 (33%), and trimethoprim-sulfamethoxazole 2/6 (33%), this shows a varying level of resistance to antibiotics. From the results of the study it can be concluded that ESBL-producing Escherichia coli bacteria were found on the hands of broiler meat traders in Al-Mahirah market, Banda Aceh city and the resistance of ESBL-producing Escherichia coli to ampicillin, cefotaxim, cefazidime, kanamycin, erythromycin, and trimethoprim-sulfamethoxazole antibiotics. Keyword: Antibiotics, ESBL, Resistance, Hand