Electronic Theses and Dissertation

Aspergillosis merupakan penyakit umum pada saluran pernapasan burung. Infeksi Aspergillus sp. ditandai dengan adanya plak atau nodul-nodul berwarna kekuningan pada saluran pernapasan yang meliputi trakea, kantung udara, dan paru-paru. Penelitian ini bertujuan untuk mengisolasi Aspergillus sp. dari paru-paru burung gereja. Sampel yang digunakan adalah paru-paru dari 35 ekor burung gereja yang diambil secara acak. Isolasi dilakukan dengan metode Thompson (1969), sampel ditanamkan pada media SDA kemudian diinkubasi selama 2-7 hari. Pertumbuhan koloni Aspergillus sp. diamati dengan interval 24 jam dengan melihat warna, bentuk, tepi, dan permukaan bawah koloni. Sementara itu untuk memastikan dan mengonfirmasi spesies Aspergillus sp. maka dilakukan penanaman jamur pada media slide culture. Setelah itu, dilakukan pengamatan secara mikroskopis dengan melihat pertumbuhan konidiofor, hifa, fialid, dan konidia jamur. Data yang diperoleh kemudian dianalisis secara deskriptif. Hasil pengamatan menunjukkan 27 dari 35 sampel positif Aspergillus sp. Berdasarkan hasil penelitian dapat disimpulkan bahwa 77,14% sampel positif Aspergillus sp. yang terdiri dari Aspergillus niger dan Aspergillus flavus.
Kata kunci : Aspergillosis, burung gereja, paru-paru

Aspergillosis is a common disease of the respiratory tract of birds. Aspergillus sp. infection is characterized by the presence of yellowish plaques or nodules in the respiratory tract which includes the trachea, air sacs, and lungs. This study aimed to isolate Aspergillus sp. from the lungs of sparrows. The samples used were the lungs of 35 sparrows taken randomly. Isolation was carried out by Thompson's method (1969), samples were embedded in SDA media and then incubated for 2-7 days. The growth of Aspergillus sp. colonies was observed at 24-hour intervals by looking at the color, shape, edges and bottom surface of the colonies. Meanwhile, to ensure and confirm the species of Aspergillus sp., the fungus was planted on slide culture media. After that, microscopic observations were made by looking at the growth of conidiophores, hyphae, phialids and fungal conidia. The data obtained were then analyzed descriptively. The results showed that 27 out of 35 samples were positive for Aspergillus sp. Based on the results of the study, it can be concluded that 77.14% of the samples were positive for Aspergillus sp. consisting of Aspergillus niger and Aspergillus flavus. Keywords: Aspergillosis, sparrow, lungs