Electronic Theses and Dissertation

Diabetes dikenal dengan the silent killer karena penyakit ini dapat menyerang semua organ tubuh sehingga menimbulkan berbagai macam keluhan bagi penderitanya. Salah satunya adalah penyebab gagal ginjal. Penelitian ini bertujuan untuk mengetahui histopatologi ginjal tikus putih model diabetes mellitus yang diberikan susu kambing fermentasi Lactobacillus casei dan filtrat daun pandan wangi (Pandanus amaryllifolius Roxb). Penelitian dilakukan menggunakan Rancangan Acak Lengkap (RAL) pola searah dengan 5 kelompok perlakuan, masing-masing terdiri atas 3 ekor tikus putih. Perlakuan 0 (P0) kontrol normal, (P1) diinjeksi streptozotocin, (P2) diinjeksi STZ dan
susu kambing fermentasi L. casei, (P3) diinjeksi STZ dan diberikan filtrat daun pandan wangi, (P4) diinjeksi STZ dan diberikan susu kambing fermentasi L. casei dan filtrat daun pandan wangi. Pemberian perlakuan dilakukan selama 14 hari dengan dosis sebanyak 2 ml tiap kelompok. Sampel ginjal tikus dikoleksi melalui prosedur nekropsi dan di euthanasia dengan cara cervical dislocation kemudian dilakukan pembuatan sediaan histopatologi. Berdasarkan hasil stastistik uji Kruskalwallis menunjukkan bahwa pemberian susu kambing fermentasi L. casei, filtrat daun pandan wangi, dan susu kambing fermentasi L. casei yang ditambah filtrat daun pandan wangi terhadap parameter
degenerasi lemak atau nekrosis tidak terdapat perbedaan yang signifikan yang ditunjukkan (P>0,05)
terhadap histopatologi ginjal tikus putih antar kelompok perlakuan. Namun rata-rata skor histopatologi ginjal kelompok perlakuan P2 (2,13 ± 0,23), P3 (2,33 ± 0,30), dan P4 (2,20 ± 0,20) lebih rendah dibandingkan kelompok perlakuan P1 (2,86 ± 0,11). Pemberian susu kambing fermentasi L. casei dan filtrat daun pandan wangi (P. amaryllifolius Roxb.) tidak berpengaruh nyata terhadap perbaikan histopatologi ginjal tikus model diabetes mellitus akan tetapi terhadap gambaran histopatologi ginjal pada tikus sudah menunjukkan perbaikan dengan adanya penurunan rata-rata tingkat kerusakan yang terlihat secara mikroskopis

Kata Kunci: Diabetes mellitus, daun pandan wangi, streptozotocin, dan susu kambing

Diabetes is known as the silent killer because this disease can attack all organs of the body, causing various complaints for sufferers. One of them isthe cause of kidney failure. This study aims to determine the histopathology of the kidneys of white rat diabetes mellitus models given Lactobacillus casei fermented goat milk and fragrant pandanusleaf filtrate (Pandanus amaryllifolius Roxb). The research was conducted using a completely randomized design (CRD) unidirectional pattern with 5 treatment groups, each consisting of 3 white rats. Treatment 0 (P0) normal control, (P1) injected streptozotocin, (P2) injected STZ and Lactobacillus casei fermented goat milk, (P3) injected STZ and given fragrant pandanus leaf filtrate, (P4) injected STZ and given L. casei fermented goat milk and fragrant pandanus leaf filtrate. Treatment was carried out for 14 days with a dose of 2 ml per group. Rat kidney samples were collected through necropsy procedures and euthanized by cervical dislocation then histopathology preparations were made. Based on the statistical results of the Kruskal-wallis test, it shows that the giving L. casei fermented goat milk, fragrant pandanus leaf filtrate, and L. casei fermented goat milk added with fragrant pandanus leaf filtrate to the parametes of fatty degeneration or necrosis, there is no significant difference shown (P>0,005) on the histopathology of rats kidneys beteween treatment groups. However, the average kidney histopathology score of treratment groups P2 (2,13 ± 0,23), P3 (2,33 ± 0,30), and P4 (2,20 ± 0,20) was lower than that treatment group P1 (2,86 ± 0,11). Giving fermented goat milk L. casei and fragrant pandanus leaf filtrate (P. amaryllifolius Roxb) does not significantly affect the improvement of kidney histopathology in diabetes mellitus model rats but the histopathological picture of the kidneys in rats has shown improvement with a decrease in the average level of damage seen microscopically. Key words : Diabetes mellitus, fragrant pandanus leaf, fermented goat milk and streptozotocin