Electronic Theses and Dissertation

Penelitian ini bertujuan untuk mengamati tingkat resistensi bakteri Escherichia coli penghasil extended spectrum β-lactamase yang terdapat pada daging ayam broiler di pasar Al-Mahirah kota Banda Aceh terhadap antibiotik. Penelitian ini menggunakan metode observasi cross sectional dengan 47 sampel daging ayam. Sampel daging ayam diisolasi untuk mendapatkan koloni bakteri Escherichia coli penghasil extended spectrum β-lactamase yang kemudian diuji resistensi dengan antibiotik sefotaksim, seftazidim, ampisilin, eritromisin, kanamisin, dan trimetoprim-sulfametoksazol menggunakan metode disc diffusion dan dianasilis secara deskriptif. Hasil penelitian menunjukan terdapat 10 sampel daging ayam positif Escherichia coli penghasil extended spectrum β-lactamase. Hasil uji resistensi pada 10 sampel tersebut terdapat 9/10 (90%) isolat Escherichia coli penghasil extended spectrum β-lactamase yang resisten terhadap sefotaksim, 9/10 (90%) isolat resisten terhadap seftazidim, 8/10 (80%) isolat resisten terhadap ampisilin, 10/10 (100%) isolat resisten terhadap eritromisin, 8/10 (80%) isolat resisten terhadap trimetoprim-sulfametoksazol, dan 3/10 (30%) isolat resisten terhadap kanamisin. Berdasarakan hasil penelitian dapat disimpulkan bahwa ditemukan sampel Escherichia coli penghasil ESBL pada daging ayam broiler di pasar Al-Mahirah kota Banda Aceh dan adanya resistensi Escherichia coli penghasil ESBL terhadap antibiotik sefotaksim, seftazidim, ampisilin, kanamisin, eritromisin, dan trimetroprim-sulfametoksazol sehingga dapat dikategorikan sebagai multidrug resistance.

This study aims to observe the resistance level of Escherichia coli producing extended-spectrum β-lactamase bacteria found in broiler chicken meat at Al-Mahirah market in Banda Aceh city to antibiotics. This study used the cross-sectional observation method on 47 chicken meat samples. Chicken meat samples were isolated to obtain colonies of Escherichia coli bacteria producing extended-spectrum β-lactamase and tested for resistance to the antibiotics cefotaxime, cefazidime, ampicillin, erythromycin, kanamycin, and trimethoprim-sulfamethoxazole using the disc diffusion method and analyzed in descriptive. The results showed there were 10 samples of chicken meat-positive Escherichia coli producing extended-spectrum β-lactamase. Resistance test results on these 10 samples, there were 9/10 (90%) isolates of Escherichia coli producing extended spectrum β-lactamase resistant to cefotaxime, 9/10 (90%) isolates resistant to cefazidime, 8/10 (80%) isolates resistant to ampicillin, 10/10 (100%) isolates resistant to erythromycin, 8/10 (80%) isolates resistant to trimethoprim-sulfamethoxazole, and 3/10 (30%) isolates resistant to kanamycin. Based on the study, it can be inferred that Escherichia coli ESBL-producing samples were detected in broiler meat at Al-Mahirah market in Banda Aceh city, and Escherichia coli ESBL-producing resistance to antibiotics cefotaxime, cefazidim, ampicillin, kanamycin, erythromycin, and trimethoprim-sulfamethoxazole can be categorized as multidrug resistance.