Electronic Theses and Dissertation

Jamur endofit yang berasosiasi pada daun Jatropha curcas Linn yang diambil di desa Meunasah Papeun, Aceh Besar, Indonesia telah berhasil diisolasi. Hasil isolasi diperoleh 5 isolat jamur endofit yang diberi nama IEDJ1, IEDJ2, IEDJ3, IEDJ4, dan IEDJ5. Hasil skrining menunjukkan bahwa hanya satu isolat yang kaya akan lipid yaitu isolat IEDJ3 dengan kandungan lipid sebesar 30,67% dari berat biomassa keringnya sehingga tergolong ke dalam mikroba oleaginous. Metil ester hasil transesterifikasi dari ekstrak lipid isolat IEDJ3 dianalisis menggunakan Gas Chromatography-Mass Spectrometry. Hasil tersebut menunjukkan bahwa metil ester asam lemak dari isolat IEDJ3 adalah metil linoleat (31,46%), metil palmitat (28,30%), metil elaidat (25,71%), metil stearat (11,53%), (Z)-7-hexadecenoic acid methyl ester (0,53%), metil miristat (0,52%), methyl 18-methylnonadecanoate (0,48%), methyl 20-methyl-heneicosanoate (0,39%), methyl 9-eicosenoate (0,35%), heneicosanoic acid, 18-propyl-, methyl ester (0,29%), benzenepropanoic acid, 3,5-bis(1,1-dimethylethyl)-4-hydroxy-, methyl ester (0,23%), dan 4,8,12,16-octadecatetraen-1-ol, 4,9,13,17-tetramethyl (0,13%). Komponen metil ester asam lemak yang diperoleh menunjukkan bahwa lipid isolat IEDJ3 dapat digunakan untuk produksi biodiesel.

Endophytic fungi associated with Jatropha curcas Linn leaves taken in Meunasah Papeun village, Aceh Besar, Indonesia have been successfully isolated. The isolation results obtained 5 isolates of endophytic fungi named IEDJ1, IEDJ2, IEDJ3, IEDJ4, and IEDJ5. Screening results show that only one isolate is rich in lipids, namely isolate IEDJ3 with a lipid content of 30.67% of its dry biomass weight so that it is classified into oleaginous microbes. Transesterified methyl esters from the lipid extract of isolate IEDJ3 were analyzed using Gas Chromatography-Mass Spectrometry. The results showed that the fatty acid methyl esters of IEDJ3 isolate were methyl linoleate (31.46%), methyl palmitate (28.30%), methyl elaidate (25.71%), methyl stearate (11.53%), (Z)-7-hexadecenoic acid methyl ester (0.53%), methyl myristate (0.52%), methyl 18-methylnonadecanoate (0, 48%), methyl 20-methyl-heneicosanoate (0.39%), methyl 9-eicosenoate (0.35%), heneicosanoic acid, 18-propyl-, methyl ester (0.29%), benzenepropanoic acid, 3,5-bis(1,1-dimethylethyl)-4-hydroxy-, methyl ester (0.23%), and 4,8,12,16-octadecatetraen-1-ol, 4,9,13,17-tetramethyl (0.13%). The fatty acid methyl ester components obtained suggest that the lipids of isolate IEDJ3 can be used for biodiesel production.