Electronic Theses and Dissertation

Telah dilakukan green synthesis nanopartikel perak dari ekstrak air daun Lantana camara sebagai antimikroba. Daun Lantana camara diambil dari daerah geotermal Ie Seu’um. Karakterisasi sampel dilakukan menggunakan Spektrofotometer UV-Vis, Foutier Transform Infra Red (FTIR), Scanning Electron Microscope-Energy Dispersive X-Ray Spectrocopy (SEM-EDS) dan Particle Siza Analyzer (PSA). Optimasi Response Surface Methodology (RSM) menunjukkan bahwa kondisi optimum pH ekstrak dan konsentrasi AgNO3 yaitu pada pH 8,6 dan konsentrasi 4,3 mM. Hasil pengukuran Spektrofotometer UV-Vis menunjukkan bahwa absorbansi maksimum pada panjang gelombang 417 nm. Hasil karakterisasi FTIR menunjukkan adanya gugus -OH, C=C, N-H dan C-H pada ekstrak dan AgNPs yang terlibat proses pembentukan nanopartikel perak. Hasil karakterisasi SEM menunjukkan bahwa rata-rata partikel AgNPs-Lc berbentuk spherical, sedangkan hasil spektrum EDS menunjukkan bahwa logam Ag merupakan logam terbanyak yang terkandung dalam AgNPs. Hasil karakterisasi PSA menunjukkan bahwa rata-rata ukuran partikel AgNPs-Lc yaitu sebesar 44 nm dengan nilai cumulative number 53,769 dan nilai differential number 24,162. Pengujian antimikroba dilakukan pada bakteri Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) dan jamur Candida albicans (C. albicans). Pengujian antimikroba terhadap AgNPs-Lc dilakukan dengan variasi konsentrasi yaitu 1%, 5%, 10% dan 20%. Hasil pengujian menunjukkan bahwa AgNPs memiliki zona hambat terhadap bakteri Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) dengan zona hambat 9,77 ± 0,13 dan 8,42 ± 0,009. Ekstrak air daun Lantana camara dari daerah geotermal Ie Seu’um dapat digunakan dalam green synthesis sebagai antibakteri.

Kata Kunci: Lantana camara, green synthesis, nanopartikel perak, AgNP-Lc, RSM dan antimikroba.

Green synthesis of silver nanoparticles has been performed using the water extract of Lantana camara leaves as an antimicrobial agent. The leaves of Lantana camara were obtained from the geothermal area of Ie Seu’um. Sample characterization was conducted using UV-Vis Spectrophotometer, Fourier Transform Infrared (FTIR), Scanning Electron Microscope-Energy Dispersive X-Ray Spectroscopy (SEM-EDS), and Particle Size Analyzer (PSA). Response Surface Methodology (RSM) optimization indicated that the optimum conditions for extract pH and AgNO3 concentration were pH 8.6 and 4.3 mM, respectively. UV-Vis Spectrophotometer measurements revealed maximum absorbance at a wavelength of 417 nm. FTIR characterization results indicated the presence of functional groups such as -OH, C=C, N-H, and C-H in both the extract and AgNPs, indicating their involvement in the silver nanoparticle formation process. SEM characterization showed that the average particles of AgNPs-Lc were spherical, while EDS spectrum results indicated that silver (Ag) was the predominant metal in AgNPs. PSA characterization demonstrated that the average particle size of AgNPs-Lc was 44 nm with a cumulative number of 53,769 and a differential number of 24,162. Antimicrobial testing was carried out against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Candida albicans (C. albicans). Antimicrobial testing on AgNPs-Lc was performed with concentrations of 1%, 5%, 10%, and 20%. The results showed that AgNPs exhibited inhibitory zones against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) with inhibition zones of 9.77 ± 0.13 and 8.42 ± 0.009, respectively. The water extract of Lantana camara leaves from the geothermal area of Ie Seu’um can be utilized in green synthesis as an antibacterial agent. Keywords: Lantana camara, green synthesis, silver nanoparticles, AgNP-Lc, RSM, Antimicrobial.