Electronic Theses and Dissertation

Kebersihan tangan merupakan faktor penting yang perlu diperhatikan dalam
mengendalikan penyebaran penyakit untuk mencegah terjadinya infeksi yang dapat
disebarkan melalui tangan. AgNP tembelekan (Lantana camara) dan AgNP paku
laut (Acrostichum aureum) yang tumbuh subur di kawasan geotermal Ie Seu-Um
dilaporkan memiliki aktivitas antibakteri. Kemampuan antibakteri yang telah
dilaporkan perlu dilakukan pengembangan sebagai produk farmasi, salah satunya
hand sanitizer. Tujuan dari penelitian ini adalah membuat hand sanitizer bebas
alkohol yang berbahan dasar AgNP tembelekan (Lantana camara) dan AgNP paku
laut (Acrostichum aureum) menggunakan metode green synthesis. Evaluasi sediaan
meliputi uji organoleptis, uji homogenitas, uji daya sebar, uji daya lekat, uji
viskositas, uji pH, uji stabilitas dipercepat dan uji iritasi. Evaluasi aktivitas
antibakteri diuji terhadap bakteri Staphylococcus aureus dan Escherichia coli.
Hasilnya menunjukkan bahwa karakteristik organoleptis keseluruhan hand sanitizer
yang diuji memiliki hasil yang baik, kecuali pada warna sediaan yang berubah
selama penyimpanan. Hasil uji pH, daya lekat, daya sebar, viskositas, homogenitas
hand sanitizer memenuhi syarat uji yang baik. Uji iritasi terhadap 10 sukarelawan
tidak menunjukkan reaksi iritasi. Uji antimikroba menunjukkan bahwa hand sanitizer
yang disiapkan memiliki aktivitas antimikroba terhadap bakteri Gram-positif dan
Gram-negatif. Pembuatan hand sanitizer berbahan dasar AgNP dari tumbuhan dapat
dijadikan hand sanitizer, namun membutuhkan penambahan stabilisator.

Hand hygiene is an important factor that needs to be considered in controlling the spread of disease to prevent infections that can be spread through hands. AgNPs of tembelekan (Lantana camara) and AgNPs of paku laut (Acrostichum aureum) thriving in the Ie Seu-Um geothermal area are reported to have antibacterial activity. The antibacterial ability that has been reported needs to be developed as a pharmaceutical product, one of which is hand sanitizer. The aim of this study was to make alcohol-free hand sanitiser based on AgNPs of tembelekan (Lantana camara) and AgNPs of paku laut (Acrostichum aureum) using green synthesis method. Evaluation of the preparation included organoleptic test, homogeneity test, spreadability test, adhesion test, viscosity test, pH test, stability test and irritation test. Activity evaluation was tested against Staphylococcus aureus and Escherichia coli bacteria. The results showed that the overall organoleptic characteristics of the tested hand sanitizers had good results, except for the colour of the preparation which changed during storage. The test results of pH, adhesion, spreadability, viscosity, homogeneity of hand sanitisers meet the requirements of a good test. Irritation test on 10 volunteers showed no irritation reaction. The antimicrobial test showed that the prepared hand sanitizer has antimicrobial activity against Grampositive and Gram-negative bacteria. AgNP-based hand sanitisers from plants can be used as hand sanitisers, but require the addition of stabilisers.